Document Detail

Transitin is required for the differentiation of avian QM7 myoblasts into myotubes.
MedLine Citation:
PMID:  20931647     Owner:  NLM     Status:  In-Process    
Transitin is a nestin-like intermediate filament protein co-expressed with vimentin in the precursor cells of the myogenic and neurogenic lineages of the avian embryo. To understand its role in myogenesis, stable cell lines expressing transitin-targeted siRNAs were derived from the quail muscle cell line QM7. When cells were cultured in differentiation medium, we found that transitin knockdown prevented myoblast fusion and myotube formation. MyoD mRNA could be detected in transitin siRNA-transfected cells, but upregulation of myogenin and desmin expression was impaired compared to control cells. In addition, transitin siRNA cells maintain high levels of Pax7 expression suggesting that QM7 myoblasts into which transitin expression has been attenuated display a muscle progenitor cell phenotype (Pax7(+)/MyoD(+)/myogenin(-)/desmin(-)). These observations indicate that transitin plays an important role in the initiation of the myogenic program in avian muscle progenitor cells in acting downstream of MyoD and upstream of myogenin during the lineage progression.
Maroua Jalouli; Louis R Lapierre; Dominique Guérette; Karine Blais; Ju-Ahng Lee; Gregory J Cole; Michel Vincent
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  239     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3038-47     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
Centre de recherche PROTEO et Département de biologie moléculaire, biochimie médicale et pathologie, Université Laval, Québec (Qc), Canada.
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Grant Support
FRN 72199//Canadian Institutes of Health Research

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