| Transit-amplifying cell frequency and cell cycle kinetics are altered in aged epidermis. | |
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MedLine Citation:
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PMID: 19458632 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aged epidermis is less proliferative than young, as exemplified by slower wound healing. However, it is not known whether quantitative and/or qualitative alterations in the stem and/or transit-amplifying (TA) compartments are responsible for the decreased proliferation. Earlier studies found a normal or decreased frequency of putative epidermal stem cells (EpiSCs) with aging. We show, using long-term repopulation in vivo and colony formation in vitro, that, although no significant difference was detected in EpiSC frequency with aging, TA cell frequency is increased. Moreover, aged TA cells persist longer, whereas their younger counterparts have already differentiated. Underlying the alteration in TA cell kinetics in the aged is an increase in the proportion of cycling keratinocytes, as well as an increase in cell cycle duration. In summary, although no significant difference in EpiSC frequency was found, TA cell frequency was increased (as measured by in vivo repopulation, growth fraction, and colony formation). Furthermore, the proliferative capacity (cellular output) of individual aged EpiSCs and TA cells was decreased compared to that of young cells. Although longer cell cycle duration contributes to the decreased proliferative output from aged progenitors, the greater number of TA cells may be a compensatory mechanism tending to offset this deficit. |
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Authors:
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Alexandra Charruyer; Chantal O Barland; Lili Yue; Heike B Wessendorf; Ying Lu; H Jeffrey Lawrence; Maria L Mancianti; Ruby Ghadially |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-05-21 |
Journal Detail:
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Title: The Journal of investigative dermatology Volume: 129 ISSN: 1523-1747 ISO Abbreviation: J. Invest. Dermatol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-14 Completed Date: 2009-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: United States |
Other Details:
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Languages: eng Pagination: 2574-83 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University of California, San Francisco, California 94121, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Cell Differentiation / physiology Cell Division / physiology Cells, Cultured Epidermis / cytology*, physiology* Flow Cytometry G1 Phase / physiology Green Fluorescent Proteins / genetics Mice Mice, Inbred C57BL Mice, SCID Mice, Transgenic S Phase / physiology Skin Aging / pathology*, physiology* Stem Cells / cytology, physiology |
| Grant Support | |
ID/Acronym/Agency:
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AG20372/AG/NIA NIH HHS; R01AR020786/AR/NIAMS NIH HHS; R03 AR48667/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
Comment In:
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J Invest Dermatol. 2009 Nov;129(11):2541-3
[PMID:
19826444
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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