Document Detail

Transient response of Enterobacter aerogenes under a dual nutrient limitation in a chemostat.
MedLine Citation:
PMID:  1252609     Owner:  NLM     Status:  MEDLINE    
Utilizing a chemostat with a dual nutrient limitation of nitrogen and phosphate, we examined the transient response of the culture following a pulse of one of the limiting nutrients (ammonia). This method provided quantitative evidence that cells can be grown under dual nutrient limitation. Furthermore, the pattern of response was consistent with the hypothesis that phosphate limitation restricts nucleic acid synthesis in the cell and that nitrogen limitation restricts protein synthesis. The net result is that under a phosphate limitation there is a restricted biosynthetic capacity which we feel is closely associated with the RNA content of the cell.
C L Cooney; D I Wang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  18     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  1976 Feb 
Date Detail:
Created Date:  1976-05-10     Completed Date:  1976-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  189-98     Citation Subset:  IM    
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MeSH Terms
Culture Media
Enterobacteriaceae / growth & development*
Nitrogen / pharmacology*
Phosphates / pharmacology*
Reg. No./Substance:
0/Culture Media; 0/Phosphates; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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