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Transient reduction of placental angiogenesis in PAI-1-deficient mice.
MedLine Citation:
PMID:  21119013     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Murine placentation is associated with the invasion of maternal endometrium by trophoblasts and an extensive maternal and fetal angiogenesis. Plasminogen activator inhibitor-1 (PAI-1) is transiently produced by spongiotrophoblasts and trophoblast giant cells at 10.5-11.5 days postcoitum (dpc). Knowing the key contribution of PAI-1 in the regulation of angiogenesis, we have now analyzed the consequence of PAI-1 deficiency on murine placentation. Morphological and quantitative computer-assisted image analysis revealed abnormal placental morphology in PAI-1(-/-) mice at 10.5 and 12.5 dpc. At 10.5 dpc, the genetic ablation of PAI-1 resulted in a transient reduction of both maternal and fetal vascularizations in the placenta and increased trophoblast cell density. This was associated with a poorer development of the labyrinth and an extension of the decidua. A larger spongiotrophoblast layer appeared at 12.5 dpc in PAI-1-deficient mice. Placental morphology was normalized at 14.5 dpc. Microarray analyses performed on laser capture microdissected labyrinths revealed that 46 genes were differentially expressed between the two genotypes at 10.5 dpc. However, only 11 genes were still differently modulated at 14.5 dpc, when normalization of placental morphology had taken place. This transcriptomic profiling highlighted a dysregulation in the expression of placenta-related cathepsin family members. Altogether our data provide evidence for a transient impaired placental morphology in PAI-1-deficient mice that is then normalized, leading to normal embryonic development.
Authors:
Soraya Labied; Silvia Blacher; Peter Carmeliet; Agnès Noël; Francis Frankenne; Jean-Michel Foidart; Carine Munaut
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Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Physiological genomics     Volume:  43     ISSN:  1531-2267     ISO Abbreviation:  Physiol. Genomics     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815683     Medline TA:  Physiol Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  188-98     Citation Subset:  IM    
Affiliation:
Laboratory of Tumour and Development Biology, University of Liège, Tour de Pathologie (B23), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Cancer (GIGA Cancer), Liège;
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