Document Detail


Transient receptor potential vanilloid type 1 (TRPV1) expression changes from normal urothelium to transitional cell carcinoma of human bladder.
MedLine Citation:
PMID:  15992990     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate possible changes in the expression of the transient receptor potential vanilloid type 1 (TRPV1) from normal urothelium to transitional cell carcinoma (TCC) of human bladder. METHODS: Specimens from normal bladder (n=13, mean age 62 yrs), superficial TCC (n=16, mean age 62,4 yrs) and muscle invasive bladder cancer (n=12, mean age 67 yrs), were obtained by multiple cold cup and full-thickness biopsy during open surgery. All the specimens were processed for H&E staining, immunohistochemistry and Western Blot analysis. RESULTS: In controls, the urothelium showed a labelling whose intensity was higher in the superficial cells than in the basal and club-shaped ones. In the superficial TCC, the urothelium showed a reduced labelling intensity. In the muscle invasive TCC, a very light labelling was occasionally detected in scattered superficial cells and no labelling was present in the basal cells and in those that had invaded the muscle. In controls, Western Blot analysis recognized two thick, intensely stained bands, with a molecular weight of approximately 100 and 95 kDa. In all superficial TCC there were two bands similar to control ones and in the muscle invasive two very thin, lightly stained bands. No band was detected in the patients staged as pT4. CONCLUSION: These data demonstrated a progressive loss of TRPV1 expression in the urothelium as TCC stage increased and cell differentiation was lower. Future studies will establish the importance of this loss for TCC differentiation and progression.
Authors:
Massimo Lazzeri; Maria Giuliana Vannucchi; Michele Spinelli; Elisa Bizzoco; Patrizia Beneforti; Damiano Turini; Maria-Simonetta Faussone-Pellegrini
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European urology     Volume:  48     ISSN:  0302-2838     ISO Abbreviation:  Eur. Urol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-13     Completed Date:  2006-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7512719     Medline TA:  Eur Urol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  691-8     Citation Subset:  IM    
Affiliation:
Department of Urology, Ospedale Santa Chiara Firenze, Firenze, Italy. lazzeri.m@tiscali.it
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MeSH Terms
Descriptor/Qualifier:
Aged
Blotting, Western
Carcinoma, Transitional Cell / chemistry*,  pathology*
Case-Control Studies
Coloring Agents
Eosine Yellowish-(YS) / diagnostic use
Female
Fluorescent Dyes
Gene Expression Regulation, Neoplastic
Hematoxylin / diagnostic use
Humans
Immunohistochemistry
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Staging
Staining and Labeling
TRPV Cation Channels / analysis*
Urinary Bladder Neoplasms / chemistry*,  pathology*
Urothelium / chemistry*,  pathology
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Fluorescent Dyes; 0/TRPV Cation Channels; 0/TRPV1 protein, human; 17372-87-1/Eosine Yellowish-(YS); 517-28-2/Hematoxylin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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