Document Detail

Transient receptor potential vanilloid 1 (TRPV1) channels in cultured rat Sertoli cells regulate an acid sensing chloride channel.
MedLine Citation:
PMID:  17945192     Owner:  NLM     Status:  MEDLINE    
Sertoli cells provide a controlled microenvironment for regulation and maintenance of spermatogenesis for which an acidic milieu is crucial for male fertility. Sertoli cells also contribute to protection of spermatogenetic cells. Here, we showed that TRPV1 is expressed in rat Sertoli cells and regulates an acid sensing Cl(-) channel (ASCC). The expression of TRPV1 in rat Sertoli cells was demonstrated by RT-PCR, immunostaining and calcium measurement experiments. ASCC activity was inhibited by capsaicin (IC(50)=214.3+/-1.6 nM), olvanil (IC(50)=400+/-1.7 pM) and resiniferatoxin (IC(50)=9.3+/-1.5 nM) but potentiated by capsazepine (EC(50)=5.3+/-1.3 microM) and ruthenium red (EC(50)=2.3+/-1.5 microM). In the human airway epithelial cell line Calu-3 in which ASCC can be detected but not TRPV1, capsaicin and capsazepine were without any effect. Finally the application of the non-steroidal anti-inflammatory drug ibuprofen prevented the control of ASCC by TRPV1. Our study provides the first evidence for a regulation by TRPV1 of an acid sensing chloride channel in rat Sertoli cells. TRPV1 and ASCC may thus be considered as new potential physiological regulators of spermatogenesis and targets for pharmacological treatments of reproductive disorders as cryptorchidism, Sertoli cell tumors or torsion of the spermatic cord.
Céline Auzanneau; Caroline Norez; Fabrice Antigny; Vincent Thoreau; Chantal Jougla; Anne Cantereau; Frédéric Becq; Clarisse Vandebrouck
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-08
Journal Detail:
Title:  Biochemical pharmacology     Volume:  75     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-28     Completed Date:  2008-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  476-83     Citation Subset:  IM    
Institut de Physiologie et Biologie Cellulaires, Université de Poitiers, CNRS, 40 avenue du recteur Pineau, 86022 Poitiers, France.
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MeSH Terms
Calcium / metabolism
Capsaicin / analogs & derivatives,  pharmacology
Cells, Cultured
Chloride Channels / physiology*
Hydrogen-Ion Concentration
Ibuprofen / pharmacology
Rats, Wistar
Sertoli Cells / metabolism*
TRPV Cation Channels / physiology*
Reg. No./Substance:
0/Chloride Channels; 0/TRPV Cation Channels; 0/Trpv1 protein, rat; 0/capsazepine; 15687-27-1/Ibuprofen; 404-86-4/Capsaicin; 7440-70-2/Calcium

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