Document Detail


Transient receptor potential ion channel TRPM7 is required for preventing replicative senescence in pancreatic adenocarcinoma cells.
MedLine Citation:
PMID:  22166235     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The transient receptor potential TRPM7 ion channel is required for cellular proliferation in pancreatic epithelia and adenocarcinoma. To elucidate the mechanism that mediates the function of TRPM7, we examined its role in survival of pancreatic cancer cells. RNA interference-mediated silencing of TRPM7 did not induce apoptotic cell death. TRPM7-deficient cells underwent replicative senescence with up-regulation of p16(CDKN2A) and WRN mRNA. The combination of anti-TRPM7 siRNA and gemcitabine produced enhanced cytotoxicity as compared to gemcitabine alone. Thus, TRPM7 is required for preventing senescence, and modulation of TRPM7 expression may help improve treatment response of pancreatic cancer by combination with apoptosis-inducing agents.
Authors:
Nelson S Yee; Weiqiang Zhou; Minsun Lee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-10
Journal Detail:
Title:  Cancer letters     Volume:  -     ISSN:  1872-7980     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ireland Ltd.
Affiliation:
Division of Hematology-Oncology, Department of Medicine, Penn State College of Medicine;Penn State Hershey Cancer Institute;Milton S. Hershey Medical Center;Pennsylvania State University, Hershey, Pennsylvania, United States of America; Division of Hematology, Oncology, and Blood & Marrow Transplantation, Department of Internal Medicine, Carver College of Medicine;Program of Cancer Signaling and Experimental Therapeutics, Holden Comprehensive Cancer Center;University of Iowa, Iowa City, Iowa, United States of America.
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