Document Detail


Transient receptor potential channel type M5 is essential for fat taste.
MedLine Citation:
PMID:  21653867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Until recently, dietary fat was considered to be tasteless, and its primary sensory attribute was believed to be its texture (Rolls et al., 1999; Verhagen et al., 2003). However, a number of studies have demonstrated the ability of components in fats, specifically free fatty acids, to activate taste cells and elicit behavioral responses consistent with there being a taste of fat. Here we show for the first time that long-chain unsaturated free fatty acid, linoleic acid (LA), depolarizes mouse taste cells and elicits a robust intracellular calcium rise via the activation of transient receptor potential channel type M5 (TRPM5). The LA-induced responses depend on G-protein-phospholipase C pathway, indicative of the involvement of G-protein-coupled receptors (GPCRs) in the transduction of fatty acids. Mice lacking TRPM5 channels exhibit no preference for and show reduced sensitivity to LA. Together, these studies show that TRPM5 channels play an essential role in fatty acid transduction in mouse taste cells and suggest that fatty acids are capable of activating taste cells in a manner consistent with other GPCR-mediated tastes.
Authors:
Pin Liu; Bhavik P Shah; Stephanie Croasdell; Timothy A Gilbertson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  31     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-09     Completed Date:  2011-09-01     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8634-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism
Fatty Acids / metabolism*
Food Preferences / physiology
Male
Mice
Mice, Knockout
Patch-Clamp Techniques
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / physiology
TRPM Cation Channels / genetics,  metabolism*
Taste / physiology*
Taste Buds / metabolism
Type C Phospholipases / metabolism
Grant Support
ID/Acronym/Agency:
DK059611/DK/NIDDK NIH HHS; R01 DC002507/DC/NIDCD NIH HHS; R01 DC002507-08/DC/NIDCD NIH HHS; R01 DC002507-09/DC/NIDCD NIH HHS; R01 DC002507-10/DC/NIDCD NIH HHS; R01 DK059611/DK/NIDDK NIH HHS; R01 DK059611-06/DK/NIDDK NIH HHS; R56 DK059611/DK/NIDDK NIH HHS; R56 DK059611-05A1/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/TRPM Cation Channels; 0/Trpm5 protein, mouse; EC 3.1.4.-/Type C Phospholipases; SY7Q814VUP/Calcium
Comments/Corrections

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