Document Detail

Transient profound mesenteric ischemia strongly affects the strength of intestinal anastomoses in the rat.
MedLine Citation:
PMID:  17205202     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Experimental data suggest that transient preoperative ischemia and reperfusion may compromise anastomotic strength. However, data on this subject are equivocal, in particular as to the onset and duration of this effect. This study was designed to comprehensively characterize the effects of profound transient intestinal ischemia on anastomotic healing during the first postoperative week. METHODS: Ischemia was induced in rats by clamping both the superior mesenteric artery and ileal branches for 30 minutes. Immediately after declamping, anastomoses were constructed in both terminal ileum and descending colon. After three, five, or seven days, both bursting pressure and breaking strength were measured. Anastomotic collagen content, gelatinase activity, and histology were analyzed. RESULTS: Anastomotic leakage rate was 13 percent in ischemia-reperfusion group and 0 percent (P=0.02) in controls. The breaking strength in ileum remained significantly (P<0.05) lower in the ischemic groups than in the control groups at all time points. Bursting pressure in the ileum was not significantly different between ischemic and control groups at either of the time points measured. However, at Day 7 the bursting site was significantly more frequent within the suture line in the ischemic groups. In the colon, at Day 3 the bursting pressure was 35 percent lower in the ischemic group than in the control group (P<0.05). Anastomotic collagen content and gelatinase activity were similar in ischemic and control groups. CONCLUSIONS: Transient profound splanchnic ischemia compromises anastomotic strength throughout the entire first postoperative week. This effect does not seem to be caused by impaired accumulation of wound collagen.
Lisanne A E Posma; Robert P Bleichrodt; Harry van Goor; Thijs Hendriks
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diseases of the colon and rectum     Volume:  50     ISSN:  0012-3706     ISO Abbreviation:  Dis. Colon Rectum     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-04     Completed Date:  2007-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372764     Medline TA:  Dis Colon Rectum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1070-9     Citation Subset:  IM    
Department of Surgery, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.
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MeSH Terms
Anastomosis, Surgical / methods
Colon / metabolism,  physiopathology,  surgery*
Disease Models, Animal
Follow-Up Studies
Gelatinases / metabolism
Hydroxyproline / metabolism
Ileum / metabolism,  physiopathology,  surgery*
Mesentery / blood supply*
Peroxidase / metabolism
Rats, Wistar
Reperfusion Injury / pathology*
Treatment Outcome
Reg. No./Substance:
51-35-4/Hydroxyproline; EC; EC 3.4.24.-/Gelatinases

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