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Transient over-expression of barley BAX Inhibitor-1 weakens oxidative defence and MLA12-mediated resistance to Blumeria graminis f.sp. hordei.
MedLine Citation:
PMID:  20507468     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
SUMMARY BAX Inhibitor-1 (BI-1) is a conserved cell death suppressor protein. In barley, BI-1 (HvBI-1) expression is induced upon powdery mildew infection and when over-expressed in epidermal cells of barley, HvBI-1 induces susceptibility to the biotrophic fungal pathogen Blumeria graminis. We co-expressed mammalian pro-apoptotic BAX together with HvBI-1, and the mammalian BAX antagonist BCL-X(L) in barley epidermal cells. BAX expression led to cessation of cytoplasmic streaming and collapse of the cytoplasm while co-expression of HvBI-1 and BCL-X(L) partially or completely, respectively, rescued cells from BAX lethality. When B. graminis was attacking epidermal cells, a green fluorescent protein fusion of HvBI-1 accumulated at the site of attempted penetration and was also present around haustoria. Over-expression of HvBI-1 in epidermal cells weakened a cell-wall-associated local hydrogen peroxide burst in a resistant mlo-mutant genotype and supported haustoria accommodation in race-specifically resistant MLA12-barley. HvBI-1 is a cell death regulator protein of barley with the potential to suppress host defence reactions.
Authors:
Ruth Eichmann; Cornelia Dechert; Karl-Heinz Kogel; Ralph Hückelhoven
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular plant pathology     Volume:  7     ISSN:  1364-3703     ISO Abbreviation:  Mol. Plant Pathol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2010-05-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954969     Medline TA:  Mol Plant Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  543-52     Citation Subset:  -    
Affiliation:
Research Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, Heinrich-Buff Ring 26-32, D-35392 Giessen, Germany.
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