Document Detail


Transient osmotic stress facilitates mutant huntingtin aggregation.
MedLine Citation:
PMID:  12499864     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human neuroblastoma SH-SY5Y cell lines stably expressing mutant truncated huntingtin with 82 (mutant) glutamine repeats (N63-82Q) were briefly exposed to hyperosmotic conditions which decrease cell volume and therefore transiently increased the concentration of N63-82Q, as well as activating specific stress-induced pathways. Transient hyperosmotic treatment significantly increased the number of cells with aggregates. When the N63-82Q cells were subsequently returned to iso-osmotic medium after the treatment, the number of cells with aggregates remained constant up to 12 h. However, between 12 and 24 h another significant increase in aggregate frequency was observed, with approximately 55% of the cells containing aggregates after 24 h. This may be due in part to the formation of microaggregates during hyperosmotic conditions that act as seeds for the aggregate formation. Further, treatment of cells with geldanamycin, which activates a heat shock response, significantly attenuated the hyperosmotic-induced increase in aggregate formation.
Authors:
Wanjoo Chun; Mathieu Lesort; Matthew Lee; Gail V W Johnson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroreport     Volume:  13     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-24     Completed Date:  2003-04-04     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  2543-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Benzoquinones
Enzyme Inhibitors / pharmacology
Heat-Shock Response / drug effects,  physiology
Humans
Hypertonic Solutions / pharmacology
Lactams, Macrocyclic
Mutagenesis / physiology
Nerve Tissue Proteins / genetics*,  metabolism*
Neuroblastoma*
Nuclear Proteins / genetics*,  metabolism*
Osmotic Pressure
Quinones / pharmacology
Tumor Cells, Cultured
Water-Electrolyte Balance / physiology*
Grant Support
ID/Acronym/Agency:
AG 12396/AG/NIA NIH HHS; NS 41552/NS/NINDS NIH HHS; R01 NS041552/NS/NINDS NIH HHS; R01 NS041552-01/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Benzoquinones; 0/Enzyme Inhibitors; 0/HTT protein, human; 0/Hypertonic Solutions; 0/Lactams, Macrocyclic; 0/Nerve Tissue Proteins; 0/Nuclear Proteins; 0/Quinones; Z3K3VJ16KU/geldanamycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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