Document Detail


Transient osmotic absorption of fluid in microvessels exposed to low concentrations of dimethyl sulfoxide.
MedLine Citation:
PMID:  16393944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dimethyl Sulfoxide (DMSO) is a common solvent for pharmacological agents. It is a small, lipophilic molecule thought to be relatively highly permeable through the cell membrane. While measuring the effect of low concentrations of DMSO (0.05-0.5% v/v) on capillary hydraulic conductivity as a vehicle control for pharmacological agents, the authors noticed what appeared to be an unusual transient absorption of fluid across the vessel wall. This absorption occurred during occlusion of the vessel, but dissipated quickly (1.7-8.6 s). The transient reabsorption reappeared upon each successive occlusion. To determine the nature of this transient absorption, the authors have measured the effect of increasing the pressure of the perfusing solution, of the concentration and time of perfusion of DMSO, and of superfusing the DMSO. They found that the absorption rate, but not the filtration rate, was concentration dependent, and was significantly correlated with the osmotic pressure of the DMSO. Moreover, the time taken for completion of the transient, i.e., time to reversal of flow, was inversely proportional to the hydraulic conductivity of the vessel. Furthermore, the transient absorption could be reduced and eventually abolished by increasing the hydrostatic pressure. These results strongly suggested that perfusion with low concentrations of DMSO could set up a significant osmotic pressure gradient across the vessel wall. This proposed mechanism for the absorption was confirmed by the measurement of a significant osmotic reflection coefficient of the vessel wall to DMSO (0.11 +/- 0.01). Relatively low concentrations (0.05-0.5%) of DMSO were therefore able to stimulate a significant osmotic transient across the blood vessel walls.
Authors:
Catherine A Glass; Rachel M Perrin; Tristan M Pocock; David O Bates
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  13     ISSN:  1073-9688     ISO Abbreviation:  Microcirculation     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-05     Completed Date:  2006-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  29-40     Citation Subset:  IM    
Affiliation:
Microvascular Research Laboratories, Department of Physiology, Preclinical Veterinary School, University of Bristol, Bristol, UK.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Animals
Capillaries
Capillary Permeability / drug effects*
Cryoprotective Agents / pharmacology*
Dimethyl Sulfoxide / pharmacology*
Dose-Response Relationship, Drug
Male
Microcirculation / drug effects
Osmosis
Osmotic Pressure / drug effects
Rana temporaria
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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