| Transient inhibition of histone deacetylase activity overcomes silencing in the mating-type region in fission yeast. | |
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MedLine Citation:
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PMID: 10079326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric, i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h90 cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species. |
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Authors:
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T G Olsson; R A Silverstein; K Ekwall; P Sunnerhagen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Current genetics Volume: 35 ISSN: 0172-8083 ISO Abbreviation: Curr. Genet. Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-06-16 Completed Date: 1999-06-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8004904 Medline TA: Curr Genet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 82-7 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Lundberg Laboratory, Göteborg University, P. O. Box 462, S-405 30 Göteborg, Sweden. Tim.Olsson@molbiol.gu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Enzyme Inhibitors
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pharmacology Fungal Proteins / genetics Gene Expression Regulation Gene Expression Regulation, Fungal / drug effects Genes, Fungal / genetics* Genes, Mating Type, Fungal* Histone Deacetylase Inhibitors* Histone Deacetylases / metabolism Hydroxamic Acids / pharmacology Recombinant Fusion Proteins / genetics Schizosaccharomyces / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Fungal Proteins; 0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 0/Recombinant Fusion Proteins; 58880-19-6/trichostatin A; EC 3.5.1.98/Histone Deacetylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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