| Transient idling of posttermination ribosomes ready to reinitiate protein synthesis. | |
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MedLine Citation:
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PMID: 15667944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fate of ribosomes between termination and initiation during protein synthesis is very basic, yet poorly understood. Here we found that translational reinitiation of the alkaline phosphatase gene occurs in Escherichia coli from an internal methionine codon when the authentic translation is prematurely terminated at a nonsense codon that is within seven codons upstream of the reinitiation codon (which we refer to as "reinitiation window"). Changing the reading frame downstream of the stop codon did not abolish the reinitiation, while inactivating the upstream initiation codon abolished the reinitiation. Moreover, depletion of the ribosome recycling factor (RRF), which disassembles posttermination ribosomes in conjunction with elongation factor G, did not influence the observed reinitiation. These findings suggest that posttermination ribosomes can undergo a transient idling state ready to reinitiate protein synthesis even in the absence of the Shine-Dalgarno (SD) sequence within the reinitiation window by evading disengagement from the mRNA. |
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Authors:
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Andrey L Karamyshev; Zemfira N Karamysheva; Tohru Yamami; Koichi Ito; Yoshikazu Nakamura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimie Volume: 86 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2005-01-25 Completed Date: 2005-06-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 933-8 Citation Subset: IM |
Affiliation:
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Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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chemistry,
genetics,
metabolism Base Sequence Codon Codon, Initiator Codon, Nonsense Codon, Terminator Escherichia coli / enzymology, genetics, metabolism Methionine / metabolism Molecular Sequence Data Mutation Open Reading Frames Peptide Chain Initiation, Translational* Peptide Chain Termination, Translational* Plasmids Protein Biosynthesis* RNA, Messenger / metabolism Ribosomes / metabolism* Sulfur Radioisotopes / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Codon; 0/Codon, Initiator; 0/Codon, Nonsense; 0/Codon, Terminator; 0/RNA, Messenger; 0/Sulfur Radioisotopes; 63-68-3/Methionine; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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