Document Detail

Transient genome-wide transcriptional response to low-dose ionizing radiation in vivo in humans.
MedLine Citation:
PMID:  17996396     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The in vivo effects of low-dose low linear energy transfer ionizing radiation on healthy human skin are largely unknown. Using a patient-based tissue acquisition protocol, we have performed a series of genomic analyses on the temporal dynamics over a 24-hour period to determine the radiation response after a single exposure of 10 cGy.
METHODS AND MATERIALS: RNA from each patient tissue sample was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array. Data analysis was performed on selected gene groups and pathways.
RESULTS: Nineteen gene groups and seven gene pathways that had been shown to be radiation responsive were analyzed. Of these, nine gene groups showed significant transient transcriptional changes in the human tissue samples, which returned to baseline by 24 hours postexposure.
CONCLUSIONS: Low doses of ionizing radiation on full-thickness human skin produce a definable temporal response out to 24 hours postexposure. Genes involved in DNA and tissue remodeling, cell cycle transition, and inflammation show statistically significant changes in expression, despite variability between patients. These data serve as a reference for the temporal dynamics of ionizing radiation response following low-dose exposure in healthy full-thickness human skin.
Susanne R Berglund; David M Rocke; Jian Dai; Chad W Schwietert; Alison Santana; Robin L Stern; Joerg Lehmann; Christine L Hartmann Siantar; Zelanna Goldberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-11-08
Journal Detail:
Title:  International journal of radiation oncology, biology, physics     Volume:  70     ISSN:  0360-3016     ISO Abbreviation:  Int. J. Radiat. Oncol. Biol. Phys.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-18     Completed Date:  2008-02-07     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  7603616     Medline TA:  Int J Radiat Oncol Biol Phys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  229-34     Citation Subset:  IM    
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MeSH Terms
Dose-Response Relationship, Radiation
Genome, Human / genetics,  radiation effects*
Linear Energy Transfer*
Oligonucleotide Array Sequence Analysis / methods
Radiation Dosage
Signal Transduction / radiation effects
Skin / pathology,  radiation effects*
Time Factors
Transcription, Genetic / radiation effects*
Grant Support
P30 CA093373-04/CA/NCI NIH HHS; P42 ES004699/ES/NIEHS NIH HHS; P42 ES004699-229005/ES/NIEHS NIH HHS; P42 ES004699-239005/ES/NIEHS NIH HHS; P42-ES04699/ES/NIEHS NIH HHS; R01 HG003352/HG/NHGRI NIH HHS; R01 HG003352-02/HG/NHGRI NIH HHS; R01 HG003352-03/HG/NHGRI NIH HHS; R01-HG003352/HG/NHGRI NIH HHS

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