| Transient expression of type II collagen and tissue mobilization during development of the scleral ossicle, a membranous bone, in the chick embryo. | |
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MedLine Citation:
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PMID: 7949369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Development of the chick scleral ossicle was studied with respect to expression of various collagen types, cartilage matrix molecules, and osteoblastic cell surface antigens. The extra-cellular matrix of the scleral ossicle primordium of stage 35.5 chick sclera and the mesenchyme beneath the conjunctival epithelium was immunoreactive with anti-type II collagen antibody, giving the impression that certain materials and/or cell clusters surrounded by reactive matrix were descending from the epithelial-mesenchymal interface to the scleral ossicle primordium. In stage 37 embryos, type II collagen immunoreactivity was restricted to the bone matrix of the scleral ossicles, and persisted through stage 39. However, at stage 41, virtually no type II collagen was detected. In contrast, strong immunostaining of type I collagen was first detected in the developing scleral ossicle at stage 37, coinciding with the formation of mineralized bone matrix. Following the extensive accumulation of type I collagen in bone matrix, type XII collagen was detected at the surface of the bone; both type I and type XII collagen immunostainings then remained. By stage 37, immunoreactivity with a pre-osteoblastic cell surface marker was detected on cells of the scleral ossicle, and typical osteocytes were subsequently identified by both morphological and specific immunostaining techniques. Antibodies other than for type II collagen, specific to chondrogenic mesenchyme or cartilage matrix, never reacted with the scleral ossicle and its primordium during development. Taken together, these observations indicate that the scleral ossicle is a membranous bone, whose development may not require overt chondrogenesis. Implications of type II collagen distribution during the positioning of scleral ossicles and their early bone matrix formation are discussed with respect to the origin and evolution of endoskeletons in vertebrate animals. |
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Authors:
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K Watanabe; S P Bruder; A I Caplan |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 200 ISSN: 1058-8388 ISO Abbreviation: Dev. Dyn. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-12-09 Completed Date: 1994-12-09 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 212-26 Citation Subset: IM; S |
Affiliation:
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Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Matrix / embryology*, metabolism Bone and Bones / embryology, metabolism* Cell Differentiation Cell Division Chick Embryo Collagen / biosynthesis* Immunohistochemistry Osteogenesis* |
| Chemical | |
Reg. No./Substance:
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9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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