Document Detail


Transient expression of β-glucuronidase following biolistic delivery of foreign DNA into coffee tissues.
MedLine Citation:
PMID:  24186705     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Some conditions related to the transient expression of β-glucuronidase in biolistically-treated Coffea spp. tissues were investigated, and subsequently used in a promoter study. Bombardments were performed on different types of tissue (leaves, somatic embryos and suspension cultures) of genotypes of C. arabica, C. canephora and Arabusta, using 4 different promoter sequences. Tobacco leaves were used as a comparison. In general, similar large variation and mean values of transient expression were observed between coffee and tobacco leaves. With regard to the coffee tissues effect, transient expression was best detectable and most frequently observed with bombarded leaves of microcuttings. Disturbing endogenous light blue staining was found with control treatments of somatic embryos. For the three coffee species tested, the most effective promoter was the EF1α-A1 promoter of Arabidopsis thaliana.
Authors:
J van Boxtel; M Berthouly; C Carasco; M Dufour; A Eskes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant cell reports     Volume:  14     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  2013-11-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  748-52     Citation Subset:  -    
Affiliation:
CIRAD-CP, B.P. 5035, F-34032, Montpellier cedex 1, France.
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