Document Detail

Transient expression of AtNCED3 and AAO3 genes in guard cells causes stomatal closure in Vicia faba.
MedLine Citation:
PMID:  18060348     Owner:  NLM     Status:  MEDLINE    
Abscisic acid (ABA) regulates stomatal closure in response to water loss. Here, we examined the competence of guard cells to synthesize ABA, using two Arabidopsis ABA biosynthetic enzymes. 35S pro::AtNCED3-GFP and AAO3-GFP were introduced into guard cells of broad bean leaves. AtNCED3-GFP expression was detected at the chloroplasts, whereas green fluorescent protein (GFP) and AAO3-GFP were in the cytosol. The stomatal aperture was decreased in AtNCED3-GFP- and AAO3-GFP-transformed guard cells. This indicated that ABA biosynthesis is stimulated by heterologous expression of AtNCED3 and Arabidopsis aldehyde oxidase 3 (AAO3) proteins, which both seem to be regulatory enzymes for ABA biosynthesis in these cells. Furthermore, stomatal closure by the expression of AtNCED3 and AAO3 suggested that the substrates of the enzymes are present and native ABA-biosynthesis enzymes are active in guard cells.
Vanessa Melhorn; Kunimi Matsumi; Hanae Koiwai; Keiichi Ikegami; Masanori Okamoto; Eiji Nambara; Florian Bittner; Tomokazu Koshiba
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Publication Detail:
Type:  Journal Article     Date:  2007-12-04
Journal Detail:
Title:  Journal of plant research     Volume:  121     ISSN:  0918-9440     ISO Abbreviation:  J. Plant Res.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-08     Completed Date:  2008-04-29     Revised Date:  2010-06-10    
Medline Journal Info:
Nlm Unique ID:  9887853     Medline TA:  J Plant Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  125-31     Citation Subset:  IM    
Department of Biological Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
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MeSH Terms
Abscisic Acid / biosynthesis
Aldehyde Oxidase / genetics,  metabolism*
Arabidopsis / genetics,  metabolism
Arabidopsis Proteins / genetics,  metabolism*
Gene Expression Regulation, Plant
Oxygenases / genetics,  metabolism*
Plant Stomata / cytology,  metabolism*
Plants, Genetically Modified
Time Factors
Vicia faba / genetics,  metabolism*
Reg. No./Substance:
0/Arabidopsis Proteins; 21293-29-8/Abscisic Acid; EC 1.13.-/Oxygenases; EC dioxygenase; EC protein, Arabidopsis; EC Oxidase

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