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Transient excretion of succinate from Trichoderma atroviride submerged mycelia reveals the complex movements and metabolism of carboxylates.
MedLine Citation:
PMID:  21337029     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Submerged growth of Trichoderma atroviride CCM F 534 on glucose-containing medium was accompanied by the excretion of organic acids (succinate, citrate, alpha-ketoglutarate, fumarate, aconitate). The excretion of succinate was transient. After 48-72 h cultivation, millimolar amounts of succinate disappeared from the medium. We studied the mechanism of the removal of succinate from the medium and demonstrated the activation of the inward transport of succinate by submerged mycelia. This transport was carrier-mediated, had a low solute specificity, and was driven by proton-motive force. The last aspect was provided by the activation of the H(+)-ATPase, as documented by measurements of ATPase activity and expression of the pma gene. The disruption of the pma gene abolished the capacity of the mycelia to re-uptake succinate but not its production. Results show that excreted carboxylates could serve as alternative nutrients in the late phase(s) of submerged growth, explain why inward transport system(s) for carboxylates are induced, and indicate that the inward-directed transport could interfere with the production of carboxylic acids by fungi.
Authors:
Petra Olejníková; Daniela Hudecová; Wolfgang Burgstaller; Svetlana Kryštofová; Ludovít Varečka
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-20
Journal Detail:
Title:  Antonie van Leeuwenhoek     Volume:  -     ISSN:  1572-9699     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372625     Medline TA:  Antonie Van Leeuwenhoek     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Biochemistry and Microbiology, Slovak University of Technology, Radlinského 9, 81237, Bratislava, Slovak Republic, petra.olejnikova@stuba.sk.
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