| Transient amidolytic activity changes of plasmin adsorbed onto carbons. | |
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MedLine Citation:
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PMID: 12202196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The amidolytic activity of plasmin (Pln) that spontaneously adsorbs from solutions to modified-graphite (GR) and glassy carbon (GL) surfaces was studied in the 10-45 degrees C temperature range in the presence of a chromogenic substrate. Surfaces were modified with a coating of fibrinogen, either electrochemically oxidized or not. The effect of an additional modification via deposition of Langmuir-Blodgett films of behenic acid (BA-LB) onto the former surfaces, thus leading to either hydrophobic or hydrophilic surfaces according to the number of deposed layers, was examined. In all cases results showed the occurrence of a first order transition which strongly and transiently increases the surface activity of Pln. At BA-LB surfaces, the most prominent change compared with other coatings was a significant enhancement of the critical temperature Tc that characterizes the beginning of the transition. When fibrinogen was present in the solution, the transition was no longer observable up to 37 degrees C as shown by the linear kinetics exhibited by surfaces bearing oxidized fibrinogen. |
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Authors:
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S Hourdin; S Longchamp; O Gallet; J-M Nigretto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomolecular engineering Volume: 19 ISSN: 1389-0344 ISO Abbreviation: Biomol. Eng. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-30 Completed Date: 2003-03-07 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100928062 Medline TA: Biomol Eng Country: Netherlands |
Other Details:
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Languages: eng Pagination: 281-5 Citation Subset: IM |
Affiliation:
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Université de Cergy-Pontoise, LECMA (EA2528), 5 mail Gay Lussac, 95031 Cergy-Pontoise Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Amides / metabolism Carbon / chemistry* Coated Materials, Biocompatible / chemistry Enzyme Activation Enzymes, Immobilized / chemistry Fatty Acids / chemistry* Fibrinogen / chemistry* Fibrinolysin / pharmacokinetics* Graphite / chemistry Materials Testing / methods Sensitivity and Specificity Substrate Specificity Surface Properties Temperature |
| Chemical | |
Reg. No./Substance:
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0/Amides; 0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 0/Fatty Acids; 112-85-6/behenic acid; 7440-44-0/Carbon; 7782-42-5/Graphite; 9001-32-5/Fibrinogen; EC 3.4.21.7/Fibrinolysin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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