Document Detail


Transient amidolytic activity changes of plasmin adsorbed onto carbons.
MedLine Citation:
PMID:  12202196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amidolytic activity of plasmin (Pln) that spontaneously adsorbs from solutions to modified-graphite (GR) and glassy carbon (GL) surfaces was studied in the 10-45 degrees C temperature range in the presence of a chromogenic substrate. Surfaces were modified with a coating of fibrinogen, either electrochemically oxidized or not. The effect of an additional modification via deposition of Langmuir-Blodgett films of behenic acid (BA-LB) onto the former surfaces, thus leading to either hydrophobic or hydrophilic surfaces according to the number of deposed layers, was examined. In all cases results showed the occurrence of a first order transition which strongly and transiently increases the surface activity of Pln. At BA-LB surfaces, the most prominent change compared with other coatings was a significant enhancement of the critical temperature Tc that characterizes the beginning of the transition. When fibrinogen was present in the solution, the transition was no longer observable up to 37 degrees C as shown by the linear kinetics exhibited by surfaces bearing oxidized fibrinogen.
Authors:
S Hourdin; S Longchamp; O Gallet; J-M Nigretto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomolecular engineering     Volume:  19     ISSN:  1389-0344     ISO Abbreviation:  Biomol. Eng.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-30     Completed Date:  2003-03-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100928062     Medline TA:  Biomol Eng     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  281-5     Citation Subset:  IM    
Affiliation:
Université de Cergy-Pontoise, LECMA (EA2528), 5 mail Gay Lussac, 95031 Cergy-Pontoise Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Amides / metabolism
Carbon / chemistry*
Coated Materials, Biocompatible / chemistry
Enzyme Activation
Enzymes, Immobilized / chemistry
Fatty Acids / chemistry*
Fibrinogen / chemistry*
Fibrinolysin / pharmacokinetics*
Graphite / chemistry
Materials Testing / methods
Sensitivity and Specificity
Substrate Specificity
Surface Properties
Temperature
Chemical
Reg. No./Substance:
0/Amides; 0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 0/Fatty Acids; 112-85-6/behenic acid; 7440-44-0/Carbon; 7782-42-5/Graphite; 9001-32-5/Fibrinogen; EC 3.4.21.7/Fibrinolysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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