Document Detail


Transgenic swine for biomedicine and agriculture.
MedLine Citation:
PMID:  12499023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Initial technologies for creating transgenic swine only permitted random integration of the construct. However, by combining the technology for homologous recombination in fetal somatic cells with that of nuclear transfer (NT), it is now possible to create specific modifications to the swine genome. The first such example is that of knocking out a gene that is responsible for hyperacute rejection (HAR) when organs from swine are transferred to primates. Because swine are widely used as models of human diseases, there are opportunities for genetic modification to alter these models or to create additional models of human disease. Unfortunately, some of the offspring resulting from NT have abnormal phenotypes. However, it appears that these abnormal phenotypes are a result of epigenetic modifications and, thus, are not transmitted to the offspring of the clones. Although the technique of producing animals with specific genetic modifications by NT has been achieved, improvements to the NT technique as well as improvements in the culture conditions for somatic cells and the techniques for genetic modification are still needed.
Authors:
R S Prather; R J Hawley; D B Carter; L Lai; J L Greenstein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Theriogenology     Volume:  59     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-12-24     Completed Date:  2003-03-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  115-23     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Published by Elsevier Science Inc.
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, 920 East Campus Drive, Columbia, MO 65211-5300, USA. prather@missouri.edu
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MeSH Terms
Descriptor/Qualifier:
Agriculture*
Animals
Animals, Genetically Modified*
Cloning, Organism / methods
Genetic Engineering*
Genetic Enhancement
Humans
Nuclear Transfer Techniques
Phenotype
Recombination, Genetic
Swine / genetics*
Tissue Donors
Transplantation, Heterologous
Grant Support
ID/Acronym/Agency:
R01 RR13438/RR/NCRR NIH HHS; R44 RR15198/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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