| Transgenic rice plants expressing a modified cry1Ca1 gene are resistant to Spodoptera litura and Chilo suppressalis. | |
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MedLine Citation:
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PMID: 19760523 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleotide sequence encoding the truncated insecticidal Cry1Ca1 protein from Bacillus thuringiensis was extensively modified based on the codon usage of rice genes. The overall G + C contents of the synthetic cry1Ca1 coding sequence were raised to 65% with an additional bias of enriching for G and C ending codons as preferred by monocots. The synthetic gene was introduced into the Chinese japonica variety, Xiushui 11, by Agrobacterium-mediated transformation. Transgenic rice plants harboring this gene were highly resistant to Chilo suppressalis and Spodoptera litura larvae as revealed by insect bioassays. High levels of Cry1Ca1 protein were obtained in the leaves of transgenic rice, which were effective in achieving 100% mortality of S. litura and C. suppressalis larvae. The levels of Cry1Ca1 expression in the leaves of these transgenic plants were up to 0.34% of the total soluble proteins. The larvae of C. suppressalis and S. litura could consume a maximum of 1.89 and 4.89 mm2 of transgenic leaf area whereas the consumption of nontransgenic leaves by these larvae was significantly higher; 58.33 and 61.22 mm2, respectively. Analysis of R1 transgenic plants indicated that the cry1Ca1 was inherited by the progeny plants and provided complete protection against C. suppressalis and S. litura larvae. |
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Authors:
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Mohsin Abbas Zaidi; Gongyin Ye; Hongwei Yao; Taek H You; Evelin Loit; Donald H Dean; Sheikh Riazuddin; Illimar Altosaar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular biotechnology Volume: 43 ISSN: 1559-0305 ISO Abbreviation: Mol. Biotechnol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-02 Completed Date: 2010-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 232-42 Citation Subset: IM |
Affiliation:
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Agricultural Biotechnology Laboratories, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Bacterial Proteins / biosynthesis, genetics*, metabolism Base Sequence Blotting, Southern Endotoxins / biosynthesis, genetics*, metabolism Hemolysin Proteins / biosynthesis, genetics*, metabolism Insecticides / metabolism, pharmacology Lepidoptera / drug effects* Molecular Sequence Data Oryza sativa / genetics, metabolism, microbiology, physiology* Plant Diseases / genetics*, microbiology Plant Leaves / metabolism Plants, Genetically Modified / genetics, metabolism, microbiology, physiology* Polymerase Chain Reaction Spodoptera / drug effects Transfection |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Endotoxins; 0/Hemolysin Proteins; 0/Insecticides; 0/insecticidal crystal protein, Bacillus Thuringiensis |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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