| Transgenic plants from shoot apical meristems of Vitis vinifera L. "Thompson Seedless" via Agrobacterium-mediated transformation. | |
| | |
MedLine Citation:
|
PMID: 17701039 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Shoot apical meristem explants of Vitis vinifera "Thompson Seedless" were used for Agrobacterium-mediated genetic transformation. It was determined that the meristems had to be subjected to a dark growth phase then wounded to obtain transgenic plants. Morphological and histological studies illustrated the role of wounding to expose apical meristem cells for transformation. A bifunctional egfp/nptII fusion gene was used to select kanamycin resistant plants that expressed green fluorescent protein (GFP). Kanamycin at a concentration of 16 mg L(-1) in selection medium resulted in recovery of non-chimeric transgenic plants that uniformly expressed GFP, whereas 8 mg L(-1) kanamycin allowed non-transgenic and/or chimeric plants to develop. Polymerase chain reaction (PCR) and Southern blot analyses confirmed the presence of transgenes and their stable integration into the genome of regenerated plants. Up to 1% of shoot tips produced stable transgenic cultures within 6 weeks of treatment, resulting in a total of 18 independent lines. |
| | |
Authors:
|
M Dutt; Z T Li; S A Dhekney; D J Gray |
Related Documents
:
|
17168889 - Spider dragline silk proteins in transgenic tobacco leaves: accumulation and field prod... 16213779 - Polymers from plants to develop biodegradable plastics. 8589939 - Paraquat tolerance of transgenic nicotiana tabacum with enhanced activities of glutathi... 22265079 - Soil contamination of toxoplasma gondii oocysts in pig farms in central china. 15687269 - Suppression of an atypically spliced rice cacta transposon transcript in transgenic pla... 15604769 - Paraquat resistance of transgenic tobacco plants over-expressing the ochrobactrum anthr... 12659539 - Interactive effects of ozone depletion and climate change on biogeochemical cycles. 9731309 - Fractions of calcium in the plant-soil system affected by the application of olive oil ... 12666819 - Disposal and degradation of pesticide waste. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-14 |
Journal Detail:
|
Title: Plant cell reports Volume: 26 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2007 Dec |
Date Detail:
|
Created Date: 2007-11-20 Completed Date: 2008-04-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
|
Languages: eng Pagination: 2101-10 Citation Subset: IM |
Affiliation:
|
Citrus Research and Education Center, University of Florida/IFAS, Lake Alfred, FL 33850, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blotting, Southern Drug Resistance / genetics Green Fluorescent Proteins / genetics, metabolism Kanamycin / pharmacology Meristem / drug effects, genetics*, ultrastructure Microscopy, Electron, Scanning Plant Shoots / drug effects, genetics*, ultrastructure Plants, Genetically Modified / drug effects, genetics*, ultrastructure Polymerase Chain Reaction Recombinant Fusion Proteins / genetics, metabolism Rhizobium / genetics Tissue Culture Techniques Transformation, Genetic Transgenes / genetics Vitis / drug effects, genetics*, ultrastructure |
| Chemical | |
Reg. No./Substance:
|
0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 59-01-8/Kanamycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The role of the glyoxylate cycle in the symbiotic fungus Tuber borchii: expression analysis and subc...
Next Document: Detection of peritoneal dissemination in gynecological malignancy: evaluation by diffusion-weighted ...