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Transgenic mice overexpressing ghrelin or ghrelin analog.
MedLine Citation:
PMID:  22975065     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
To understand the chronic effects of ghrelin, genetically engineered mouse models would be useful. Early studies, however, suggested that it was challenging to generate ghrelin gain-of-activity models by standard procedures. Although several groups have been trying to generate transgenic (Tg) mice overexpressing ghrelin, almost all these animals produced only des-acyl ghrelin rather than acylated ghrelin. Therefore, to elucidate the mechanism for the fatty acid modification in ghrelin, many researchers have been seeking an enzyme that would catalyze the acylation of ghrelin with an octanoic acid. In 2008, ghrelin O-acyltransferase (GOAT) was identified at last, and thereafter double-Tg mice overexpressing ghrelin and GOAT were generated by Kirchner et al. On the other hand, we have succeeded in generating Tg mice overexpressing Trp(3)-ghrelin, a ghrelin analog that does not require posttranscriptional modification with GOAT for activity. These ghrelin gain-of-activity models are useful tools for evaluating the long-term pathophysiological and/or pharmacological effects of ghrelin or ghrelin analogs and provide insight into the physiological roles of ghrelin/GHS-R systems.
Authors:
Hiroyuki Ariyasu; Go Yamada; Hiroshi Iwakura; Takashi Akamizu; Kenji Kangawa; Kazuwa Nakao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  514     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  371-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Endocrinology and Metabolism, Kyoto University Graduate School of Medicine, Kyoto, Japan; Ghrelin Research Project of Translational Research Center, Kyoto University Graduate School of Medicine, Kyoto, Japan. Electronic address: ariyasu@kuhp.kyoto-u.ac.jp.
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