Document Detail


Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis.
MedLine Citation:
PMID:  20012190     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. However, no evident morphological abnormalities were observed. Thus, overexpression of Ptmaa alone is not sufficient to cause disorganized growths or even cancer in zebrafish skin. Molecular and histological evidences showed that Tg(k18:Ptmaa-RFP) embryos have more proliferating cells in the pelvic fins [WT: 3.92 +/- 7.15; Tg(k18:Ptmaa-RFP): 38.00 +/- 10.87] and thicker skin [WT: 10.98 +/- 1.41 mum; Tg(k18:Ptmaa-RFP): 14.02 +/- 1.32 mum], indicating that overexpression of Ptmaa can promote proliferation. On the other hand, fewer apoptotic signals were found when Tg(k18:Ptmaa-RFP) embryos were exposed to UVB. Together with quantitative RT-PCR data, we suggest that UVB-induced epidermal cell apoptosis of zebrafish larvae can be attenuated by overexpression of Ptmaa through the enhancement of transcriptions of bcl2 mRNAs. Taken together, we conclude that overexpression of Ptmaa in zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis but does not cause skin cancer.
Authors:
Chiung-Wen Pai; Yau-Hung Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-11
Journal Detail:
Title:  Transgenic research     Volume:  19     ISSN:  1573-9368     ISO Abbreviation:  Transgenic Res.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-13     Completed Date:  2010-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9209120     Medline TA:  Transgenic Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  655-65     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Tamkang University, Tamsui, Taiwan ROC.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Apoptosis / genetics,  radiation effects*
Carcinoma / genetics,  pathology
Cell Proliferation* / radiation effects
Embryo, Nonmammalian
Epidermis / metabolism*,  physiology,  radiation effects
Gene Expression / physiology
Gene Transfer Techniques
Protein Precursors / genetics*,  metabolism,  physiology
Skin Neoplasms / genetics,  pathology
Thymosin / analogs & derivatives*,  genetics,  metabolism,  physiology
Ultraviolet Rays*
Up-Regulation / genetics,  radiation effects
Zebrafish / embryology,  genetics*,  metabolism,  physiology
Chemical
Reg. No./Substance:
0/Protein Precursors; 0/prothymosin alpha; 61512-21-8/Thymosin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparative efficacy of bromocriptine, cabergoline and thyroxine in inducing oestrus in bitches.
Next Document:  Changes in freezing tolerance in hybrid poplar caused by up- and down-regulation of PtFAD2 gene expr...