Document Detail


Transgenic chickens.
MedLine Citation:
PMID:  23278121     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The development of transgenic chicken technology has lagged far behind that of mammalian species. Two reasons for this are that only a one-cell-stage oocyte can be obtained from a sacrificed hen and that the yolk prevents high-magnification microscopic observation of oocytes. Recently, several new methods have been developed that will enable the successful establishment of transgenic chickens. Retroviral vectors are used in many cases because of their ability to incorporate transgenes into host cell chromosomes in a highly efficient manner. These viral vectors are injected directly into the embryos, usually at the blastodermal stage. In some cases, primordial germ cells (PGCs) are infected in vitro and then implanted into recipient embryos. Methods that do not rely on retroviral vectors are also available for creating transgenic chickens. Long-term culture of PGCs permits the selection of stably transfected cells and implantation of the manipulated PGCs. In addition, embryonic stem (ES) cell systems are available; however, the induction of functional gametes from ES cells has not, to our knowledge, been successful. It is clear that recent developments suggest that chickens may be used as a valuable experimental genetic system.
Authors:
Ken-Ichi Nishijima; Shinji Iijima
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-26
Journal Detail:
Title:  Development, growth & differentiation     Volume:  -     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Development, Growth & Differentiation © 2012 Japanese Society of Developmental Biologists.
Affiliation:
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
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