| Transgenetic studies implicate interactions between homologous PrP isoforms in scrapie prion replication. | |
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MedLine Citation:
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PMID: 1977523 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transgenic (Tg) mice expressing both Syrian hamster (Ha) and mouse (Mo) prion protein (PrP) genes were used to probe the mechanism of scrapie prion replication. Four Tg lines expressing HaPrP exhibited distinct incubation times ranging from 48 to 277 days, which correlated inversely with HaPrP mRNA and HaPrPC. Bioassays of Tg brain extracts showed that the prion inoculum dictates which prions are synthesized de novo. Tg mice inoculated with Ha prions had approximately 10(9) ID50 units of Ha prions per gram of brain and less than 10 units of Mo prions. Conversely, Tg mice inoculated with Mo prions synthesized Mo prions but not Ha prions. Similarly, Tg mice inoculated with Ha prions exhibited neuropathologic changes characteristic of hamsters with scrapie, while Mo prions produced changes similar to those in non-Tg mice. Our results argue that species specificity of scrapie prions resides in the PrP sequence and prion synthesis is initiated by a species-specific interaction between PrPSc in the inoculum and homologous PrPC. |
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Authors:
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S B Prusiner; M Scott; D Foster; K M Pan; D Groth; C Mirenda; M Torchia; S L Yang; D Serban; G A Carlson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell Volume: 63 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1990 Nov |
Date Detail:
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Created Date: 1990-12-24 Completed Date: 1990-12-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 673-86 Citation Subset: IM |
Affiliation:
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Department of Neurology, University of California, San Francisco 94143. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Blotting, Western Brain / metabolism, microbiology, pathology Cricetinae Enzyme-Linked Immunosorbent Assay Mesocricetus Mice Mice, Transgenic Nerve Degeneration PrPSc Proteins Prions / genetics*, physiology RNA, Messenger / genetics Transcription, Genetic Viral Proteins / genetics* Virus Replication* |
| Grant Support | |
ID/Acronym/Agency:
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AG02132/AG/NIA NIH HHS; NS14069/NS/NINDS NIH HHS; NS22786/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/PrPSc Proteins; 0/Prions; 0/RNA, Messenger; 0/Viral Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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