Document Detail


Transgenetic studies implicate interactions between homologous PrP isoforms in scrapie prion replication.
MedLine Citation:
PMID:  1977523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transgenic (Tg) mice expressing both Syrian hamster (Ha) and mouse (Mo) prion protein (PrP) genes were used to probe the mechanism of scrapie prion replication. Four Tg lines expressing HaPrP exhibited distinct incubation times ranging from 48 to 277 days, which correlated inversely with HaPrP mRNA and HaPrPC. Bioassays of Tg brain extracts showed that the prion inoculum dictates which prions are synthesized de novo. Tg mice inoculated with Ha prions had approximately 10(9) ID50 units of Ha prions per gram of brain and less than 10 units of Mo prions. Conversely, Tg mice inoculated with Mo prions synthesized Mo prions but not Ha prions. Similarly, Tg mice inoculated with Ha prions exhibited neuropathologic changes characteristic of hamsters with scrapie, while Mo prions produced changes similar to those in non-Tg mice. Our results argue that species specificity of scrapie prions resides in the PrP sequence and prion synthesis is initiated by a species-specific interaction between PrPSc in the inoculum and homologous PrPC.
Authors:
S B Prusiner; M Scott; D Foster; K M Pan; D Groth; C Mirenda; M Torchia; S L Yang; D Serban; G A Carlson
Related Documents :
24480303 - Inadequate reinforcement of transmedial disruptions at branch points subtends aortic an...
23211623 - Intratumoral delivery of low doses of anti-cd40 mab combined with monophosphoryl lipid ...
18025243 - Tweak/fn14 interactions are instrumental in the pathogenesis of nephritis in the chroni...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  63     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-24     Completed Date:  1990-12-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  673-86     Citation Subset:  IM    
Affiliation:
Department of Neurology, University of California, San Francisco 94143.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Blotting, Western
Brain / metabolism,  microbiology,  pathology
Cricetinae
Enzyme-Linked Immunosorbent Assay
Mesocricetus
Mice
Mice, Transgenic
Nerve Degeneration
PrPSc Proteins
Prions / genetics*,  physiology
RNA, Messenger / genetics
Transcription, Genetic
Viral Proteins / genetics*
Virus Replication*
Grant Support
ID/Acronym/Agency:
AG02132/AG/NIA NIH HHS; NS14069/NS/NINDS NIH HHS; NS22786/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/PrPSc Proteins; 0/Prions; 0/RNA, Messenger; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Crystal structure of an engrailed homeodomain-DNA complex at 2.8 A resolution: a framework for under...
Next Document:  Regulatory role of microfilaments in the induction of T4 cell proliferation and interleukin 2 produc...