| Transgenerational effect of fetal programming on vascular phenotype and reactivity in endothelial nitric oxide synthase knockout mouse model. | |
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MedLine Citation:
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PMID: 18771972 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The objective of the study was to investigate the transgenerational effect of fetal vascular programming STUDY DESIGN: Homozygous NOS3 knockout and wild type controls (NOS3+/+WT) were cross-bred to obtain heterozygous offspring that developed in (KO-/-) mothers lacking a functional NOS3 (KOM) vs wild-type control mothers (KOP). The first-generation KOM(+/-) and KOP(+/-) female mice were then bred with WT(+/+) males to obtain a second generation (F2). F2 offspring were genotyped and WT(+/+)-F2 mice were then used for in vivo blood pressure and in vitro vascular reactivity studies. RESULTS: WT-F2 mice born to KOM mothers (KOM-F2WT) had significantly higher systolic blood pressure, mean arterial pressure, and pulse pressure, compared with WT-F2 born to KOP mothers. Male KOM-F2WT offspring had significantly increased response to phenylephrine (PE), compared with male KOP-F2WT. Male offspring had increased contractile responses to PE when compared with female. Acetylcholine responses were decreased in female KOM-F2WT, compared with female KOP-F2WT, but the difference was not statistically significant CONCLUSION: Our findings support a transgenerational effect of fetal programming on the vascular phenotype and suggest possible gender specific adaptation. |
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Authors:
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Maged M Costantine; Labib M Ghulmiyyah; Esther Tamayo; Gary D V Hankins; George R Saade; Monica Longo |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 199 ISSN: 1097-6868 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-05 Completed Date: 2008-10-30 Revised Date: 2011-04-01 |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 250.e1-7 Citation Subset: AIM; IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX 77555-0144, USA. mmcostan@utmb.edu. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Blood Pressure / genetics, physiology* Body Weight Female Fetal Development / genetics, physiology* Male Mice Mice, Knockout NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase / deficiency Phenotype Vasoconstriction / genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL080558-02/HL/NHLBI NIH HHS; R01 HL080558-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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