Document Detail

Transgene repeat arrays interact with distant heterochromatin and cause silencing in cis and trans.
MedLine Citation:
PMID:  9383061     Owner:  NLM     Status:  MEDLINE    
Tandem repeats of Drosophila transgenes can cause heterochromatic variegation for transgene expression in a copy-number and orientation-dependent manner. Here, we demonstrate different ways in which these transgene repeat arrays interact with other sequences at a distance, displaying properties identical to those of a naturally occurring block of interstitial heterochromatin. Arrays consisting of tandemly repeated white transgenes are strongly affected by proximity to constitutive heterochromatin. Moving an array closer to heterochromatin enhanced variegation, and enhancement was reverted by recombination of the array onto a normal sequence chromosome. Rearrangements that lack the array enhanced variegation of white on a homologue bearing the array. Therefore, silencing of white genes within a repeat array depends on its distance from heterochromatin of the same chromosome or of its paired homologue. In addition, white transgene arrays cause variegation of a nearby gene in cis, a hallmark of classical position-effect variegation. Such spreading of heterochromatic silencing correlates with array size. Finally, white transgene arrays cause pairing-dependent silencing of a non-variegating white insertion at the homologous position.
D R Dorer; S Henikoff
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetics     Volume:  147     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-02-05     Completed Date:  1998-02-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1181-90     Citation Subset:  IM    
Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
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MeSH Terms
ATP-Binding Cassette Transporters*
Drosophila / genetics*
Drosophila Proteins*
Eye Proteins*
Gene Expression Regulation
Genes, Insect
Genes, Reporter
Insect Proteins / genetics*
Linkage (Genetics)
Repetitive Sequences, Nucleic Acid*
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Drosophila Proteins; 0/Eye Proteins; 0/Heterochromatin; 0/Insect Proteins; 0/white protein, Drosophila

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