| Transgene-mediated suppression of the RNA interference pathway in Aedes aegypti interferes with gene silencing and enhances Sindbis virus and dengue virus type 2 replication. | |
| | |
MedLine Citation:
|
PMID: 23331493 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
RNA interference (RNAi) is the major innate antiviral pathway in Aedes aegypti that responds to replicating arboviruses such as dengue virus (DENV) and Sindbis virus (SINV). On the one hand, the mosquito's RNAi machinery is capable of completely eliminating DENV2 from Ae. aegypti. On the other, transient silencing of key genes of the RNAi pathway increases replication of SINV and DENV2, allowing the viruses to temporally overcome dose-dependent midgut infection and midgut escape barriers (MEB) more efficiently. Here we expressed Flock house virus B2 (FHV-B2) from the poly-ubiquitin (PUb) promoter in Ae. aegypti using the ΦC31 site-directed recombination system to investigate the impact of transgene-mediated RNAi pathway suppression on infections with SINV-TR339eGFP and DENV2-QR94, the latter of which has been shown to be confronted with a strong MEB in Ae. aegypti. FHV-B2 was constitutively expressed in midguts of sugar- and blood-fed mosquitoes of transgenic line PUbB2 P61. B2 over-expression suppressed RNA silencing of carboxypeptidase A-1 (AeCPA-1) in midgut tissue of PUbB2 P61 mosquitoes. Following oral challenge with SINV-TR339eGFP or DENV2-QR94, mean titres in midguts of PUbB2 P61 females were significantly higher at 7 days post-bloodmeal (pbm) than in those of nontransgenic control mosquitoes. At 14 days pbm, infection rates of carcasses were significantly increased in PubB2 P61 mosquitoes infected with SINV-TR339eGFP. Following infection with DENV2-QR94, midgut infection rates were significantly increased in the B2-expressing mosquitoes at 14 days pbm. However, B2 expression in PUbB2 P61 did not increase the DENV2-QR94 dissemination rate, indicating that the infection phenotype was not primarily controlled by RNAi. |
| | |
Authors:
|
C C H Khoo; J B Doty; M S Heersink; K E Olson; A W E Franz |
Related Documents
:
|
22922903 - Dengue: an emerging disease in nepal. 23269383 - Massive mortality of prussian carp carassius gibelio in the upper elbe basin associated... 22079423 - Spatio-temporal epidemiology of highly pathogenic avian influenza (h5n1) outbreaks in n... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Insect molecular biology Volume: 22 ISSN: 1365-2583 ISO Abbreviation: Insect Mol. Biol. Publication Date: 2013 Feb |
Date Detail:
|
Created Date: 2013-01-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9303579 Medline TA: Insect Mol Biol Country: England |
Other Details:
|
Languages: eng Pagination: 104-14 Citation Subset: IM |
Copyright Information:
|
© 2013 Royal Entomological Society. |
Affiliation:
|
Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Variable induction of vitellogenin genes in the varroa mite, Varroa destructor (Anderson & Trueman),...
Next Document: Dangerous prey and daring predators: a review.