| Transfusion-induced enhancement of prostaglandin and thromboxane release in prospective kidney graft recipients. | |
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MedLine Citation:
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PMID: 3887386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pre-transplant blood transfusions (BT) improve the survival of kidney grafts. Apart from specific immunoregulation by T suppressor cells or anti-idiotypic antibodies, the role of non-specific immunoregulatory factors, such as prostaglandins is being discussed as a possible mechanism for this effect. We studied the in vitro prostanoid release from peripheral mononuclear cells following three deliberate blood transfusions. Twenty-five previously non-transfused dialysis patients were studied. Spontaneous and LPS-induced prostaglandin E (PGE) and thromboxane B2 (TXB2) were determined in cell-free culture supernatants by fluid phase RIA. Transfused patients exhibited a more rapid onset and steeper increase of prostanoid production. After 24 hours incubation, the spontaneous and LPS-induced PGE release of pre- and post-BT cells was significantly different (pre-BT: 2.1 and 5.1 ng/ml; post-3-BT: 5.0 and 7.9 ng/ml; p less than 0.01). Pre-BT cells released considerably lower amounts of TXB2 than post-BT cells (spontaneous release: 39 vs 88 ng/ml; LPS-induced release: 62 ng/ml vs 129 ng/ml; p less than 0.05). After correction for monocytes as defined by monoclonal antibodies, post-BT cells again showed increased prostanoid release as compared to pre-BT cells. Therefore, the enhanced PGE and TXB2 release of post-BT cells is not caused by an increase merely in the number of monocytes. Rather, BT appear to induce an enhanced release of prostanoids by activation of monocytes. We also found a correlation between the number of BT and the amount of prostanoid release. |
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Authors:
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V Lenhard; G Maassen; G Opelz |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Proceedings of the European Dialysis and Transplant Association - European Renal Association. European Dialysis and Transplant Association - European Renal Association. Congress Volume: 21 ISSN: 0308-9401 ISO Abbreviation: Proc Eur Dial Transplant Assoc Eur Ren Assoc Publication Date: 1985 |
Date Detail:
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Created Date: 1985-06-19 Completed Date: 1985-06-19 Revised Date: 2008-02-26 |
Medline Journal Info:
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Nlm Unique ID: 8503947 Medline TA: Proc Eur Dial Transplant Assoc Eur Ren Assoc Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 923-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Blood Transfusion* Child Female Graft Survival Humans Kidney Transplantation* Lipopolysaccharides / pharmacology Male Prostaglandins E / secretion Renal Dialysis Thromboxane B2 / secretion |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/Prostaglandins E; 54397-85-2/Thromboxane B2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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