Document Detail

Transfusion of cryopreserved human red blood cells into healthy humans is associated with rapid extravascular hemolysis without a proinflammatory cytokine response.
MedLine Citation:
PMID:  22612879     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Transfusion of stored red blood cells (RBCs) can be associated with adverse side effects. Recent studies in mice transfused with stored RBCs showed that a strong proinflammatory cytokine storm was induced due to extravascular hemolysis already at 2 hours after transfusion. Therefore, we here investigated if transfusion of 2 units of cryopreserved autologous RBCs induced a proinflammatory response in healthy human volunteers.
STUDY DESIGN AND METHODS: Two units of autologous RBCs, cryopreserved for 16 weeks, were transfused into 10 healthy human volunteers. Serum and blood samples taken at 2 hours before and at 2 and 48 hours after transfusion were analyzed for signs of extravascular hemolysis and the presence of proinflammatory cytokines.
RESULTS: At 2 hours after transfusion, transferin-bound serum iron, as well as transferin saturation and total bilirubin, were already significantly increased. These measures all returned back toward that in pretransfusion samples at 48 hours after transfusion. No increases in the production of the proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, monocyte chemotactic protein-1, macrophage inflammatory protein-1β, or tumor necrosis factor-α were detected at any time point after transfusion.
CONCLUSION: Although a significant level of extravascular hemolysis already occurred at 2 hours after transfusion of cryopreserved RBCs, there were no signs of proinflammatory cytokine production up to 48 hours after transfusion.
Andreas Hult; Christer Malm; Per-Arne Oldenborg
Related Documents :
2301709 - Susceptibility of the western fence lizard (sceloporus occidentalis) to the lyme borrel...
1918409 - Problems of thermonuclease detection for identifying staphylococcus aureus in blood cul...
7649199 - Use of ribotyping to investigate tracheal colonisation by staphylococcus epidermidis as...
24339189 - Quantitative measurement of blood velocity in zebrafish with optical vector field tomog...
15174789 - Whole blood interleukin 8 and plasma interleukin 8 levels in newborn infants with suspe...
2232629 - Successful therapy of meningococcal sepsis in acute disseminated lupus erythematosus wi...
3199139 - Total blood loss in major joint arthroplasty. a comparison of cemented and noncemented ...
7088679 - Direct measurements of oxygen tension at the surface of arterioles, capillaries and ven...
19142189 - Increased intravascular flow rate triggers cerebral arteriogenesis.
Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-21
Journal Detail:
Title:  Transfusion     Volume:  53     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-03-14     Revised Date:  2013-09-05    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28-33     Citation Subset:  IM    
Copyright Information:
© 2012 American Association of Blood Banks.
Department of Integrative Medical Biology, Section for Histology and Cell Biology, Umeå University, Umeå, SE-90187, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chemokine CCL2
Cytokines / metabolism*
Erythrocyte Transfusion / adverse effects*
Erythrocytes / cytology*,  metabolism*
Hemolysis / physiology*
Interleukin-10 / metabolism
Interleukin-1beta / metabolism
Interleukin-6 / metabolism
Interleukin-8 / metabolism
Tumor Necrosis Factor-alpha / metabolism
Young Adult
Reg. No./Substance:
0/Chemokine CCL2; 0/Cytokines; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10
Comment In:
Transfusion. 2013 Jul;53(7):1620-1   [PMID:  23844644 ]
Transfusion. 2013 Jan;53(1):5-7   [PMID:  23294207 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Biochemical and growth acclimation of birch to night temperatures: genotypic similarities and differ...
Next Document:  The changes in female physical and childbearing characteristics in China and potential association w...