Document Detail


Transfusion of cryopreserved human red blood cells into healthy humans is associated with rapid extravascular hemolysis without a proinflammatory cytokine response.
MedLine Citation:
PMID:  22612879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Transfusion of stored red blood cells (RBCs) can be associated with adverse side effects. Recent studies in mice transfused with stored RBCs showed that a strong proinflammatory cytokine storm was induced due to extravascular hemolysis already at 2 hours after transfusion. Therefore, we here investigated if transfusion of 2 units of cryopreserved autologous RBCs induced a proinflammatory response in healthy human volunteers.
STUDY DESIGN AND METHODS: Two units of autologous RBCs, cryopreserved for 16 weeks, were transfused into 10 healthy human volunteers. Serum and blood samples taken at 2 hours before and at 2 and 48 hours after transfusion were analyzed for signs of extravascular hemolysis and the presence of proinflammatory cytokines.
RESULTS: At 2 hours after transfusion, transferin-bound serum iron, as well as transferin saturation and total bilirubin, were already significantly increased. These measures all returned back toward that in pretransfusion samples at 48 hours after transfusion. No increases in the production of the proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, monocyte chemotactic protein-1, macrophage inflammatory protein-1β, or tumor necrosis factor-α were detected at any time point after transfusion.
CONCLUSION: Although a significant level of extravascular hemolysis already occurred at 2 hours after transfusion of cryopreserved RBCs, there were no signs of proinflammatory cytokine production up to 48 hours after transfusion.
Authors:
Andreas Hult; Christer Malm; Per-Arne Oldenborg
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-21
Journal Detail:
Title:  Transfusion     Volume:  53     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-03-14     Revised Date:  2013-09-05    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28-33     Citation Subset:  IM    
Copyright Information:
© 2012 American Association of Blood Banks.
Affiliation:
Department of Integrative Medical Biology, Section for Histology and Cell Biology, Umeå University, Umeå, SE-90187, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adult
Chemokine CCL2
Cryopreservation*
Cytokines / metabolism*
Erythrocyte Transfusion / adverse effects*
Erythrocytes / cytology*,  metabolism*
Hemolysis / physiology*
Humans
Interleukin-10 / metabolism
Interleukin-1beta / metabolism
Interleukin-6 / metabolism
Interleukin-8 / metabolism
Male
Tumor Necrosis Factor-alpha / metabolism
Young Adult
Chemical
Reg. No./Substance:
0/Chemokine CCL2; 0/Cytokines; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10
Comments/Corrections
Comment In:
Transfusion. 2013 Jul;53(7):1620-1   [PMID:  23844644 ]
Transfusion. 2013 Jan;53(1):5-7   [PMID:  23294207 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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