| Transfusion-associated immunomodulation: Quantitative changes in cytokines as a measure of immune responsiveness after one time blood transfusion in neurosurgery patients. | |
| | |
MedLine Citation:
|
PMID: 20859504 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Very few studies in humans have investigated the laboratory evidences suggestive of transfusion-associated immunologic changes. In this prospective study, we examined the effects of perioperative blood transfusion on immune response, by measuring various cytokines production, namely, interferon-gamma (IFN-γ), interleukin-10 (IL-10), and Fas Ligand (FasL). A total of 40 patients undergoing neurosurgery were randomly allocated into four groups: (a) no transfusion, (b) allogeneic non-leukofiltered transfusion, (c) prestorage leukofiltered transfusion, (d) autologous transfusion. Samples were collected before operation (day 0) and postoperative days (post-op) 1, 7, and 14. IFN-γ and IL-10 production capacity was measured in supernatant after whole blood culture and serum FasL levels in patients' sera using commercially available ELISA kits. Change in ratios (cytokine value after PHA stimulation/control value) of IFN-γ and IL-10 and percentage change from baseline for serum FasL levels across different transfusion groups during the sampling period were calculated. There was an increase in IL-10 production in patients receiving allogeneic non-leukofiltered transfusion on days 1 and 7 (mean ratio 2.22 (± 2.16), 4.12 (± 1.71), 4.46 (± 1.97) on days 0, 1, and 7, respectively). Similarly there was a significant (P<0.05) decrease in IFN-γ production in patients who received allogeneic non-leukofiltered red cell transfusion on post-op days 1, 7, and 14 (mean ratio 6.88 (± 4.56), 2.53 (± 0.95), 3.04 (± 1.38) and 2.58 (± 1.48) on day 0, 1, 7, and 14, respectively). Serum FasL production was increased across all patients till 7th day except for 'no transfusion' group and this increase was most significant in the non-leukofiltered group. We conclude that one time transfusion leads to quantitative changes in levels of these cytokines largely through interplay of Th2/Th1 pathways in allogeneic nonleukofiltered blood transfusion; however, soluble mediators like FasL which are also present in autologous and leukofiltered blood products may contribute toward minor immunologic effect in these settings. |
| | |
Authors:
|
Prashant Pandey; Rajendra Chaudhary; Amita Aggarwal; Raj Kumar; Dheeraj Khetan; Anupam Verma |
Related Documents
:
|
3984004 - Unnecessary blood transfusions in elective colorectal cancer surgery. 9253784 - Emerging trends in surgical blood transfusion. 879204 - Hematologic problems in pregnancy v. obstetric transfusion therapy. 15847674 - Fatal yersinia enterocolitica biotype 4 serovar o:3 sepsis after red blood cell transfu... 9479064 - Blood flow increases linearly in rat somatosensory cortex with increased whisker moveme... 15734624 - Broad-beam spectral doppler sonification of the vena contracta using matrix-array techn... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Asian journal of transfusion science Volume: 4 ISSN: 1998-3565 ISO Abbreviation: Asian J Transfus Sci Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-09-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101306858 Medline TA: Asian J Transfus Sci Country: India |
Other Details:
|
Languages: eng Pagination: 78-85 Citation Subset: - |
Affiliation:
|
Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transfusion-transmitted parasitic infections.
Next Document: Standardization of B19 IgG ELISA to study the seroepidemiology of parvovirus B19 in North Indian vol...