| Transforming potential of the c-fms proto-oncogene (CSF-1 receptor). | |
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MedLine Citation:
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PMID: 3027579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The c-fms proto-oncogene encodes a transmembrane glycoprotein that is probably identical to the receptor for the macrophage colony stimulating factor, CSF-1. Forty C-terminal amino acids of the normal receptor are replaced by 11 unrelated residues in the feline v-fms oncogene product, deleting a C-terminal tyrosine residue (Tyr969) whose phosphorylation might negatively regulate the receptor kinase activity. We show that the human c-fms gene stimulates growth of mouse NIH 3T3 cells in agar in response to human recombinant CSF-1, indicating that receptor transduction is sufficient to induce a CSF-1 responsive phenotype. Although cells transfected with c-fms genes containing either Tyr969 or Phe969 were not transformed, cotransfection of these genes with CSF-1 complementary DNA induced transformation, with c-fms(Phe969) showing significantly more activity than c-fms(Tyr969). In the absence of CSF-1, chimaeric v-fms/c-fms genes encoding the wild-type c-fms C terminus were poorly transforming, whereas chimaeras bearing Phe969 were as transforming as v-fms. Thus, the Phe969 mutation, although not in itself sufficient to induce transformation, activates the oncogenic potential of c-fms in association with an endogenous ligand or in conjunction with mutations elsewhere in the c-fms gene that confer ligand-independent signals for growth. |
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Authors:
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M F Roussel; T J Dull; C W Rettenmier; P Ralph; A Ullrich; C J Sherr |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nature Volume: 325 ISSN: 0028-0836 ISO Abbreviation: Nature Publication Date: 1987 Feb 5-11 |
Date Detail:
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Created Date: 1987-03-24 Completed Date: 1987-03-24 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0410462 Medline TA: Nature Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 549-52 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cell Line Cell Transformation, Neoplastic / genetics Colony-Stimulating Factors Fibroblasts Glycoproteins / genetics, physiology Humans Membrane Proteins / genetics, physiology Mice Oncogene Protein gp140(v-fms) Phosphorylation Proto-Oncogene Proteins / genetics, physiology* Receptor, Macrophage Colony-Stimulating Factor Receptors, Cell Surface / genetics, physiology* Receptors, Colony-Stimulating Factor Recombinant Proteins / genetics, pharmacology Retroviridae Proteins / genetics Sequence Homology, Nucleic Acid Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA 38187/CA/NCI NIH HHS; RR-05584/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Colony-Stimulating Factors; 0/Glycoproteins; 0/Membrane Proteins; 0/Oncogene Protein gp140(v-fms); 0/Proto-Oncogene Proteins; 0/Receptors, Cell Surface; 0/Receptors, Colony-Stimulating Factor; 0/Recombinant Proteins; 0/Retroviridae Proteins; EC 2.7.10.1/Receptor, Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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