| Transforming lepidopteran insect cells for improved protein processing. | |
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MedLine Citation:
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PMID: 17951779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lepidopteran insect cells used with the baculovirus expression vector system (BEVS) are capable of synthesizing and accurately processing foreign proteins. However, proteins expressed in baculovirus-infected cells often fail to be completely processed, or are not processed in a manner that meets a researcher's needs. This chapter discusses a metabolic engineering approach that addresses this problem. Basically, this approach involves the addition of new or enhancement of existing protein processing functions in established lepidopteran insect cell lines. Methods for engineering these cell lines and assessing their properties as improved hosts for the BEVS are detailed. Examples of lepidopteran insect cell lines engineered for improved protein N-glycosylation and trafficking are described. |
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Authors:
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Robert L Harrison; Donald L Jarvis |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 388 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-10-22 Completed Date: 2007-11-13 Revised Date: 2013-04-10 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 341-56 Citation Subset: IM |
Affiliation:
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USDA/ARS Insect Biocontrol Laboratory, Beltsville, MD, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Baculoviridae / genetics Cell Line Glycosylation Lepidoptera / cytology, genetics, metabolism* Models, Genetic Protein Engineering / methods Protein Processing, Post-Translational* Recombinant Proteins / genetics, metabolism* Transformation, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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GM49734/GM/NIGMS NIH HHS; R01 GM049734/GM/NIGMS NIH HHS; R01 GM049734-12/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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