Document Detail


Transforming lepidopteran insect cells for improved protein processing.
MedLine Citation:
PMID:  17951779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lepidopteran insect cells used with the baculovirus expression vector system (BEVS) are capable of synthesizing and accurately processing foreign proteins. However, proteins expressed in baculovirus-infected cells often fail to be completely processed, or are not processed in a manner that meets a researcher's needs. This chapter discusses a metabolic engineering approach that addresses this problem. Basically, this approach involves the addition of new or enhancement of existing protein processing functions in established lepidopteran insect cell lines. Methods for engineering these cell lines and assessing their properties as improved hosts for the BEVS are detailed. Examples of lepidopteran insect cell lines engineered for improved protein N-glycosylation and trafficking are described.
Authors:
Robert L Harrison; Donald L Jarvis
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  388     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-22     Completed Date:  2007-11-13     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  341-56     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Baculoviridae / genetics
Cell Line
Glycosylation
Lepidoptera / cytology,  genetics,  metabolism*
Models, Genetic
Protein Engineering / methods
Protein Processing, Post-Translational*
Recombinant Proteins / genetics,  metabolism*
Transformation, Genetic
Grant Support
ID/Acronym/Agency:
GM49734/GM/NIGMS NIH HHS; R01 GM049734/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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