Document Detail


Transforming growth factors (TGF-alpha and TGF-beta1) in the determination of vitality and wound age: immunohistochemical study on human skin wounds.
MedLine Citation:
PMID:  16139107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In continuation of former investigations on proinflammatory cytokines, in the present study the relevance of the transforming growth factors TGF-alpha and TGF-beta1 was evaluated for the diagnosis of vitality and wound age. Paraffin sections from human skin wounds due to sharp force influence, which had been collected in operations and autopsies, were investigated using immunohistochemistry. The wound age varied from a few minutes to a maximum of 6 weeks with focus on the early post-traumatic interval up to 5h. Samples from uninjured skin were available as controls. TGF-alpha (n=74) was weakly expressed in normal skin and showed a marked increase in epidermal reactivity after a wound age of approximately 10 min. The maximum was between 30 and 60 min. TGF-beta1 (n=51) revealed constitutional expression only in connective tissue. An increase of immunohistochemical reaction was partially detected even in classical stab wounds (wound age of several minutes). The immunohistochemically detectable signal concerned--presumably due to an infiltration with TGF-beta-rich thrombocytes--large parts of the traumatized skin and also the epidermal layers (cellular and interstitial marking). TGF-beta1 peaked after a post-traumatic interval of 30-60 min. Both factors, especially TGF-beta1, remained detectable in elevated levels also in older wounds with an age of days to weeks (network in granulation tissue). TGF-alpha and TGF-beta1 can efficiently contribute to the estimation of vitality and wound age based on the evaluation of cytokine patterns. In particular, this applies to TGF-beta1 because of its easier evaluation and rapid up-regulation. Similar to other cytokines, the parallel investigation of control skin from the same individual must be recommended to eliminate variation in the basal expression.
Authors:
W Grellner; S Vieler; B Madea
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Publication Detail:
Type:  Journal Article     Date:  2004-11-18
Journal Detail:
Title:  Forensic science international     Volume:  153     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-05     Completed Date:  2005-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  174-80     Citation Subset:  IM    
Affiliation:
Institute of Forensic Medicine, University of Mainz, Am Pulverturm 3, D-55131 Mainz, Germany. grellner@uni-mainz.de
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / metabolism
Blood Platelets / metabolism
Case-Control Studies
Connective Tissue / metabolism
Forensic Pathology*
Humans
Immunohistochemistry
Skin / injuries*,  metabolism*,  pathology
Time Factors
Transforming Growth Factors / metabolism*
Up-Regulation
Chemical
Reg. No./Substance:
0/Biological Markers; 76057-06-2/Transforming Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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