Document Detail

Transforming growth factor-beta3 stimulates lactotrope cell growth by increasing basic fibroblast growth factor from folliculo-stellate cells.
MedLine Citation:
PMID:  10698159     Owner:  NLM     Status:  MEDLINE    
Recently, we have shown that transforming growth factor-beta3 (TGFbeta3) mediates estradiol's mitogenic action in primary cultures of mixed anterior pituitary cells. In some cell types, TGFbeta isoforms stimulate cell proliferation via a paracrine mechanism by increasing growth stimulatory peptide growth factors. Whether such a mechanism exists in pituitary cell culture was examined in the studies presented here. The data demonstrate that unlike the response of lactotropes in mixed pituitary cultures, cultures of enriched lactotropes, obtained by Percoll gradient separation, did not proliferate in response to TGFbeta3 treatment. The lactotropic cells of the RC-4B/C cell line, a cell line that contains all of the hormone-secreting cell types of the anterior pituitary but is devoid of folliculo-stellate (FS) cells, did not proliferate in response to TGFbeta3 unless RC-4B/C cells were cocultured with FS cells. Enriched lactotropes cocultured with FS cells also demonstrated a proliferative response to TGFbeta3. Media collected from FS cells treated with TGFbeta3 stimulated the proliferation of lactotropes in enriched cultures. TGFbeta3 increased the release of basic fibroblast growth factor from FS cells. Immunoneutralization of basic fibroblast growth factor in FS cell-conditioned medium inhibited the growth stimulatory action on lactotropes. These data provide evidence for a novel mechanism of TGFbeta3 action involving cell-to-cell interaction in the anterior pituitary between lactotropes and FS cells during estrogen-induced mitogenesis.
S Hentges; N Boyadjieva; D K Sarkar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  141     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-03-16     Completed Date:  2000-03-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  859-67     Citation Subset:  AIM; IM    
Department of Veterinary and Comparative Anatomy, Washington State University, Pullman 99164-6520, USA.
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MeSH Terms
Antimetabolites / diagnostic use
Bromodeoxyuridine / diagnostic use
Cell Division / drug effects
Cell Line
Cells, Cultured
Estradiol / pharmacology
Fibroblast Growth Factor 2 / metabolism*
Paracrine Communication / physiology
Pituitary Gland, Anterior / cytology*,  drug effects,  metabolism*
Prolactin / metabolism
Rats, Inbred F344
Transforming Growth Factor beta / pharmacology*
Grant Support
Reg. No./Substance:
0/Antimetabolites; 0/Transforming Growth Factor beta; 103107-01-3/Fibroblast Growth Factor 2; 50-28-2/Estradiol; 59-14-3/Bromodeoxyuridine; 9002-62-4/Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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