Document Detail

Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation.
MedLine Citation:
PMID:  16245315     Owner:  NLM     Status:  MEDLINE    
In order to test the hypothesis that transforming growth factor beta (TGF-beta) acts by FS regulation on bovine granulosa cells in in vitro differentiation, we analyzed the effect of TGF-beta1 on follistatin mRNA expression in three differentiation states of bovine granulosa cells. We showed a positive regulation of FS mRNA after TGF-beta1 (1 ng/ml) treatment of freshly isolated granulosa cells from small-medium antral follicles (2-8 mm). This effect was abolished by the addition of exogenous follistatin (100 ng/ml), suggesting that this effect could be mediated by activin. Although these cells showed a similar effect on FS mRNA expression after treatment with activin-A, a soluble form of activin receptor type IIA was unable to inactivate the TGF-beta effect. When we tested the TGF-beta effect on FS mRNA in different granulosa cell states, TGF-beta1 regulation was associated with progesterone production only in freshly isolated cells. The amount of total activin-A produced by first passage cells (dedifferentiated cells), was ten times smaller than the one measured in a conditioned medium from freshly isolated cells (mature cells). The TGF-beta1-dependent FS mRNA expression persisted in first passage cells without changes with FS addition. On the other hand, the BGC-1 granulosa cell line (immature cells) produced large amounts of activin-A regulated by TGF-beta1 and an invariable steady state of FS mRNAs. In summary, our results showed that FS mRNA expression is regulated by TGF-beta1 independently of activin effects in differentiated granulosa cells.
Monica Fazzini; Griselda Vallejo; Alejandro Colman-Lerner; Romina Trigo; Stella Campo; J Lino S Barañao; Patricia E Saragüeta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  207     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-01-31     Completed Date:  2006-06-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  40-8     Citation Subset:  IM    
Instituto de Biologia y Medicina Experimental-CONICET, Dto de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
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MeSH Terms
Activin Receptors, Type II / pharmacology
Activins / metabolism,  pharmacology
Binding, Competitive
Cell Differentiation / genetics*
Cell Line
Cells, Cultured
Culture Media, Conditioned / chemistry
Fibronectins / metabolism
Follistatin / genetics*,  pharmacology
Gene Expression / drug effects
Granulosa Cells / cytology,  drug effects*,  metabolism
Inhibin-beta Subunits / metabolism,  pharmacology
Progesterone / metabolism
Protein-Serine-Threonine Kinases
RNA, Messenger / genetics,  metabolism*
Receptors, Transforming Growth Factor beta / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transforming Growth Factor beta / metabolism,  pharmacology*
Transforming Growth Factor beta1
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Fibronectins; 0/Follistatin; 0/RNA, Messenger; 0/Receptors, Transforming Growth Factor beta; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 0/activin A; 104625-48-1/Activins; 57-83-0/Progesterone; 93443-12-0/Inhibin-beta Subunits; EC Kinases; EC Receptors, Type II; EC receptor type II-A; EC growth factor-beta type II receptor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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