| Transforming growth factor beta: A role in the upper airway and rhinosinusitis-Dermatophagoides pteronyssinus-induced apoptosis with pulmonary alveolar cells. | |
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MedLine Citation:
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PMID: 21819759 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: There is a link with the upper and lower airway and disruption of alveolar epithelial cells, which is a potential trigger for the reactivation of the epithelial-mesenchymal trophic unit (EMTU) and induced characteristic airway changes associated with allergic asthma. Dermatophagoides pteronyssinus is a common inhalant indoor allergen and is known for causing allergic rhinitis and airway inflammation. Transforming growth factor beta 1 (TGF-beta1) is a major participant in the airway remodeling of asthma, a component of cellular stress response pathways, and enhanced epithelial immunoreactivity is known to occur in allergic rhinitis. METHODS: In this study, we show the ability of D. pteronyssinus allergens from dialyzed standardized immunotherapy extract to induce apoptosis and increase TGF-beta1 secretion in a confluent A549 cell line model. A549 cells were treated with either 600 AU/mL dialyzed D. pteronyssinus immunotherapy extract (eDp) or Ctl media (Ctl) for 24 hours. Cells and supernatants were collected, washed, and treated with Annexin V-FITC Apoptosis Detection Kit II (BD Pharmingen, La Jolla, CA) and then analyzed by flow cytometry. TGF-beta1 secretion was determined by ELISA using cell culture supernatants. RESULTS: The eDp group showed a fourfold increase in early apoptotic cells with a twofold increase in late apoptotic cells versus the Ctl group, along with a 1.65-fold increase of TGF-beta1. CONCLUSION: eDp induced viable A549 cells to undergo apoptosis determined by flow cytometry analysis with a significant increase in TGF-beta1 secretion compared with Ctl. |
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Authors:
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Patrick D Frisella; Jonathan Silverberg; Rauno Joks; Marianne Frieri |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of rhinology & allergy Volume: 25 ISSN: 1945-8932 ISO Abbreviation: Am J Rhinol Allergy Publication Date: 2011 Jul-Aug |
Date Detail:
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Created Date: 2011-08-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101490775 Medline TA: Am J Rhinol Allergy Country: United States |
Other Details:
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Languages: eng Pagination: 231-5 Citation Subset: IM |
Affiliation:
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University of New York Downstate Medical Center, Center for Allergy and Asthma Research, Brooklyn, New York, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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