Document Detail


Transforming growth factor beta regulation of migration in wounded rat intestinal epithelial monolayers.
MedLine Citation:
PMID:  8514065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In vitro studies have suggested that transforming growth factor beta 1 (TGF-beta 1) plays an important role in the regulation of proliferation of intestinal epithelial cells, effecting strong inhibition of proliferation in intestinal epithelial cell lines. Studies were undertaken to assess its role in repair after injury using an in vitro wounding model. METHODS: Wounds were created in confluent monolayers of the intestinal epithelial cell line of IEC-6. Exogenous TGF-beta 1, conditioned media from wounded IEC-6 cultures, or control media were added. Restitution was quantified as the number of cells migrating across the wound edge. Proliferation was assessed by uptake of bromodeoxyuridine and thymidine incorporation. RESULTS: Although TGF-beta was a potent inhibitor of proliferation, it promoted rapid "healing" of the monolayers through stimulation of migration of cells across the wound margin. The physiological importance of this activity was supported by the demonstration that conditioned medium from IEC-6 cells stimulated repair of the wounded monolayers. Effects of the conditioned medium could be entirely blocked by immunoneutralizing anti-TGF-beta antisera. Further, addition of protease inhibitors (aprotinin, epsilon-aminocaproic acid) that prevented the bioactivation of latent TGF-beta secreted by the IEC-6 cells also ablated the effect of the conditioned medium. In addition, expression of TGF-beta 1 messenger RNA was significantly enhanced in the wounded monolayers. CONCLUSIONS: These findings suggest that TGF-beta may play an important role in reconstitution of epithelial integrity after mucosal injury.
Authors:
C Ciacci; S E Lind; D K Podolsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gastroenterology     Volume:  105     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-07-22     Completed Date:  1993-07-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  93-101     Citation Subset:  AIM; IM    
Affiliation:
Gastrointestinal Unit, Massachusetts General Hospital, Boston.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects
Cell Line
Cell Movement / drug effects
Culture Media, Conditioned
Epithelium / drug effects
Fibrinolysin / physiology
Intestines / drug effects*,  physiology
Rabbits
Rats
Swine
Transforming Growth Factor beta / pharmacology*
Wound Healing*
Grant Support
ID/Acronym/Agency:
P30DK43351/DK/NIDDK NIH HHS; R01DK41557/DK/NIDDK NIH HHS; R01HL42457/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Transforming Growth Factor beta; EC 3.4.21.7/Fibrinolysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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