Document Detail

Transforming growth factor-beta protein and messenger RNA expression is increased in the closing ductus arteriosus.
MedLine Citation:
PMID:  8929862     Owner:  NLM     Status:  MEDLINE    
In full-term newborns, permanent closure of the ductus arteriosus is associated with the formation of a neointima that is characterized by extracellular matrix deposition and smooth muscle cell migration. Transforming growth factor-beta (TGF-beta), a potent modulator of extracellular matrix deposition and smooth muscle cell migration, has been found to play a role in the remodeling associated with several forms of vascular disease. We examined the protein and mRNA expression of the three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2, and TGF-beta3) during ductus arteriosus closure in full-term lambs. We found that the temporal changes and cellular localization of the proteins and mRNAs of all three TGF-beta isoforms were similar. TGF-beta proteins and mRNAs were present in very low levels in the late-gestation fetal ductus. Within 24 h of delivery, there was enhanced expression of TGF-beta in the newly forming neointima and outer muscle media; this continued to increase over the next 10 d. Increased expression of TGF-beta in the inner muscle media and adventitia lagged behind that of the neointima and outer muscle media. TGF-beta was not found in the luminal endothelial cells at any time. In contrast to the pattern described above, the appearance of TGF-beta protein differed from that of mRNA in the vasa vasorum of the ductus wall. After delivery, there was an increase in TGF-beta immunoreactivity in the smooth muscle cell layers of the vasa vasorum without any concurrent mRNA expression. The appearance of TGF-beta at the time of ductus closure suggests an important role for this growth factor in the reorganization of the ductus wall after birth.
J E Tannenbaum; N S Waleh; F Mauray; L Gold; E A Perkett; R I Clyman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pediatric research     Volume:  39     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1997-02-06     Completed Date:  1997-02-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  427-34     Citation Subset:  IM    
Cardiovascular Research Institute, University of California, San Francisco 94143-0544, USA.
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MeSH Terms
Ductus Arteriosus / embryology,  metabolism*,  ultrastructure
Gene Expression
Immunoenzyme Techniques
In Situ Hybridization
RNA, Messenger / metabolism
Transforming Growth Factor beta / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 0/Transforming Growth Factor beta

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