Document Detail


Transforming growth factor-beta controls receptor levels for epidermal growth factor in NRK fibroblasts.
MedLine Citation:
PMID:  6319010     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NRK fibroblasts exposed to transforming growth factor-beta (TGF-beta) show increased binding of radiolabeled epidermal growth factor (EGF) relative to untreated cells. The binding of another growth factor, rat insulin-like growth factor-II, is unaffected. The increase in EGF binding induced by TGF-beta is not due to inhibition of EGF processing nor to an alteration in the affinity of plasma membrane EGF receptors. However, treatment of the cells with TGF-beta does cause a rapid increase in the number of plasma membrane receptors for EGF. TGF-beta has little effect on the rate of overall protein synthesis, but the increase it induces in EGF binding can be completely inhibited by cycloheximide and tunicamycin. Thus a selective synthetic mechanism underlies TGF-beta action. Cells incubated with TGF-beta also show altered down regulation of their EGF receptors in response to the ligand; concentrations of EGF that can induce strong biological responses no longer decrease the plasma membrane receptor level below the basal state. These results agree well with the known specificity and synergism of the interaction between TGF-beta and EGF. Moreover, they describe a mechanism of growth control in which bioactive peptides act coordinately through a regulatory effect on the number of cell-surface receptors.
Authors:
R K Assoian; C A Frolik; A B Roberts; D M Miller; M B Sporn
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  36     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-03-02     Completed Date:  1984-03-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  35-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cycloheximide / pharmacology
Epidermal Growth Factor / metabolism*
Growth Substances / pharmacology*
Kinetics
Peptides / pharmacology*
Protein Biosynthesis
Rats
Receptor, Epidermal Growth Factor
Receptors, Cell Surface / drug effects*,  metabolism
Transforming Growth Factors
Tunicamycin / pharmacology
Chemical
Reg. No./Substance:
0/Growth Substances; 0/Peptides; 0/Receptors, Cell Surface; 11089-65-9/Tunicamycin; 62229-50-9/Epidermal Growth Factor; 66-81-9/Cycloheximide; 76057-06-2/Transforming Growth Factors; EC 2.7.10.1/Receptor, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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