Document Detail

Transforming growth factor beta activates Rac1 and Cdc42Hs GTPases and the JNK pathway in skeletal muscle cells.
MedLine Citation:
PMID:  12566226     Owner:  NLM     Status:  MEDLINE    
The transforming growth factor beta (TGFbeta) plays an important role in cell growth and differentiation. However, the intracellular signaling pathways through which TGFbeta inhibits skeletal myogenesis remain largely undefined. By measuring GTP-loading of Rho GTPases and the organization of the F-actin cytoskeleton and the plasma membrane, we analyzed the effect of TGFbeta addition on the activity of three GTPases, Rac1, Cdc42Hs and RhoA. We report that TGFbeta activates Rac1 and Cdc42Hs in skeletal muscle cells, two GTPases previously described to inhibit skeletal muscle cell differentiation whereas it inactivates RhoA, a positive regulator of myogenesis. We further show that TGFbeta activates the C-jun N-terminal kinases (JNK) pathway in myoblastic cells through Rac1 and Cdc42Hs GTPases. We propose that the activation of Rho family proteins Rac1 and Cdc42Hs which subsequently regulate JNK activity participates in the inhibition of myogenesis by TGFbeta.
Mayya Meriane; Sophie Charrasse; Franck Comunale; Cécile Gauthier-Rouvière
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  94     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2003-02-04     Completed Date:  2003-11-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  France    
Other Details:
Languages:  eng     Pagination:  535-43     Citation Subset:  IM    
Centre de recherche de biochimie macromoléculaire (CRBM), CNRS UPR 1086, IFR24, 1919, route de Mende, 34293 cedex, Montpellier, France.
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MeSH Terms
Cell Differentiation
Cell Line
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases / metabolism*
Muscle Cells / metabolism
Muscle Development
Muscle, Skeletal / cytology,  metabolism
Protein-Tyrosine Kinases / metabolism*
Transforming Growth Factor beta / physiology*
rac1 GTP-Binding Protein / metabolism*
rho GTP-Binding Proteins / metabolism
Reg. No./Substance:
0/Transforming Growth Factor beta; EC 2.7.1.-/Cdc42Hs-associated kinase; EC Kinases; EC Mitogen-Activated Protein Kinases; EC Protein Kinases; EC GTP-Binding Protein; EC GTP-Binding Proteins

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