Document Detail

Transforming growth factor-alpha: identification in bovine corpus luteum by immunohistochemistry and northern blot analysis.
MedLine Citation:
PMID:  8190906     Owner:  NLM     Status:  MEDLINE    
Transforming growth factor-alpha (TGF-alpha), a product of the thecal cells, has potent mitogenic and steroidogenic influences on cells within the ovarian follicle. Whether TGF-alpha continues to be produced in those follicles that go on to ovulate and form a corpus luteum is currently under investigation. In the present study, TGF-alpha was localized in the bovine corpus luteum by means of immunoperoxidase staining using a monoclonal antibody for TGF-alpha that does not cross-react with epidermal growth factor. In corpora lutea from the mid-luteal phase of the cycle TGF-alpha staining was found predominantly in the large luteal cells. Northern blot analysis using a human TGF-alpha cDNA probe hybridized to the 4.5-4.8 kb TGF-alpha transcript in RNA from the corpus luteum. These studies provide new evidence that TGF-alpha, a potent paracrine regulator within the ovarian follicle, continues to be expressed in the corpus luteum.
D K Lobb; J H Dorrington
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  5     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  1993  
Date Detail:
Created Date:  1994-06-23     Completed Date:  1994-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  523-9     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, McMaster University, Hamilton, Ontario, Canada.
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MeSH Terms
Blotting, Northern
Corpus Luteum / chemistry*
Transforming Growth Factor alpha / analysis*
Reg. No./Substance:
0/Transforming Growth Factor alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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