Document Detail

Transforming growth factor (TGF)-beta1 releasing tricalcium phosphate/chitosan microgranules as bone substitutes.
MedLine Citation:
PMID:  15553224     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Tricalcium phosphate (TCP)/chitosan composite microgranules were developed as bone substitutes and tissue engineering scaffolds with the aim of obtaining a high bone forming efficacy. The microgranules have the ability to fill various types of defect sites with closer packing. In addition, the transforming growth factor-beta 1 (TGF-beta1) was added to the microgranules in order to improve bone-healing efficacy. METHODS: TCP/chitosan microgranules were fabricated by dropping a TCP suspended chitosan solution into a NaOH/ethanol solution. TGF-beta1 was incorporated into the TCP/chitosan microgranules by soaking the microgranules into the TGF-beta1 solution. Scanning electron microscopy (SEM) observations as well as experiments examining the release of TGF-beta1 from chitosan and TCP/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules, and the extent of cell proliferation was evaluated using a dimethyl-thiazol tetrazolium bromide (MTT) assay. The differentiated cell function was assessed by measuring the alkaline phosphatase activity as well as performing an osteocalcin assay. RESULTS: The size of the prepared microgranules was 350-500 microm and TCP powders were observed on the surface of the microgranules. TGF-beta1 was released from the TCP/chitosan microgranules at a therapeutic concentration for 4 weeks. The proliferation of osteoblasts on the TGF-beta1 loaded microgranules was the highest among the microgranules. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer fashion. The ALPase activity and osteocalcin content of all the samples increased during the culture period. CONCLUSIONS: These results suggest that the TCP/chitosan microgranules are potential bone substitutes with a drug releasing capacity and a osteoblastic cells culture scaffold.
Jue-Yeon Lee; Yang-Jo Seol; Kyoung-Hwa Kim; Yong-Moo Lee; Yoon-Jeong Park; In-Chul Rhyu; Chong-Pyoung Chung; Seung-Jin Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pharmaceutical research     Volume:  21     ISSN:  0724-8741     ISO Abbreviation:  Pharm. Res.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-11-19     Completed Date:  2005-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1790-6     Citation Subset:  IM    
College of Pharmacy, Ewha Womans University, 11-1 Daehyundong, Seodaemun-Ku, Seoul 120-750, South Korea.
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MeSH Terms
3T3 Cells
Alkaline Phosphatase / analysis,  metabolism
Bone Substitutes / pharmacology*
Calcium Phosphates / pharmacology*
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Chitosan / pharmacology*
Microscopy, Electron, Scanning
Osteocalcin / biosynthesis,  metabolism
Tetrazolium Salts
Tissue Engineering
Transforming Growth Factor beta / metabolism*
Transforming Growth Factor beta1
Reg. No./Substance:
0/Bone Substitutes; 0/Calcium Phosphates; 0/Powders; 0/Tetrazolium Salts; 0/Tgfb1 protein, mouse; 0/Thiazoles; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 104982-03-8/Osteocalcin; 298-93-1/thiazolyl blue; 7758-87-4/tricalcium phosphate; 9012-76-4/Chitosan; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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