| Transforming growth factor (TGF)-beta1 releasing tricalcium phosphate/chitosan microgranules as bone substitutes. | |
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MedLine Citation:
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PMID: 15553224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Tricalcium phosphate (TCP)/chitosan composite microgranules were developed as bone substitutes and tissue engineering scaffolds with the aim of obtaining a high bone forming efficacy. The microgranules have the ability to fill various types of defect sites with closer packing. In addition, the transforming growth factor-beta 1 (TGF-beta1) was added to the microgranules in order to improve bone-healing efficacy. METHODS: TCP/chitosan microgranules were fabricated by dropping a TCP suspended chitosan solution into a NaOH/ethanol solution. TGF-beta1 was incorporated into the TCP/chitosan microgranules by soaking the microgranules into the TGF-beta1 solution. Scanning electron microscopy (SEM) observations as well as experiments examining the release of TGF-beta1 from chitosan and TCP/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules, and the extent of cell proliferation was evaluated using a dimethyl-thiazol tetrazolium bromide (MTT) assay. The differentiated cell function was assessed by measuring the alkaline phosphatase activity as well as performing an osteocalcin assay. RESULTS: The size of the prepared microgranules was 350-500 microm and TCP powders were observed on the surface of the microgranules. TGF-beta1 was released from the TCP/chitosan microgranules at a therapeutic concentration for 4 weeks. The proliferation of osteoblasts on the TGF-beta1 loaded microgranules was the highest among the microgranules. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer fashion. The ALPase activity and osteocalcin content of all the samples increased during the culture period. CONCLUSIONS: These results suggest that the TCP/chitosan microgranules are potential bone substitutes with a drug releasing capacity and a osteoblastic cells culture scaffold. |
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Authors:
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Jue-Yeon Lee; Yang-Jo Seol; Kyoung-Hwa Kim; Yong-Moo Lee; Yoon-Jeong Park; In-Chul Rhyu; Chong-Pyoung Chung; Seung-Jin Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pharmaceutical research Volume: 21 ISSN: 0724-8741 ISO Abbreviation: Pharm. Res. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-11-19 Completed Date: 2005-03-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406521 Medline TA: Pharm Res Country: United States |
Other Details:
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Languages: eng Pagination: 1790-6 Citation Subset: IM |
Affiliation:
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College of Pharmacy, Ewha Womans University, 11-1 Daehyundong, Seodaemun-Ku, Seoul 120-750, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Alkaline Phosphatase / analysis, metabolism Animals Bone Substitutes / pharmacology* Calcium Phosphates / pharmacology* Cell Differentiation / drug effects Cell Proliferation / drug effects Cell Survival / drug effects Chitosan / pharmacology* Mice Microscopy, Electron, Scanning Microspheres Osteocalcin / biosynthesis, metabolism Powders Tetrazolium Salts Thiazoles Tissue Engineering Transforming Growth Factor beta / metabolism* Transforming Growth Factor beta1 |
| Chemical | |
Reg. No./Substance:
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0/Bone Substitutes; 0/Calcium Phosphates; 0/Powders; 0/Tetrazolium Salts; 0/Tgfb1 protein, mouse; 0/Thiazoles; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 104982-03-8/Osteocalcin; 298-93-1/thiazolyl blue; 7758-87-4/tricalcium phosphate; 9012-76-4/Chitosan; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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