| Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways. | |
| | |
MedLine Citation:
|
PMID: 23275087 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-β1 (TGF-β1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD). Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal-regulated kinase 1/2(ERK1/2) or NF-κB pathways is able to suppress aneurysm formation. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure. Thus, we investigated the molecular mechanisms underlying TGF-β1-induced MMP-9 expression in VSMC, the involvement of intracellular ROS and signaling molecules, including ERK1/2 and NF-κB. Rat vascular smooth muscle cells (A7r5) were used. MMP-9 expression was analyzed by gelatin zymography, western blot and RT-PCR. The involvement of intracellular ROS and signaling molecules including ERK1/2 and NF-κB in the responses was investigated using reactive oxygen scavenger N-acetylcysteine (NAC) and pharmacological inhibitors (U0126 and BAY11-7082), determined by ROS testing and western blot testing for their corresponding proteins. TGF-β1 induces MMP-9 expression via ROS-dependent signaling pathway. ROS production leads to activation of ERK1/2 and then activation of the NF-κB transcription factor. Activated NF-κB turns on transcription of the MMP-9 gene. The process in which TGF-β1 induces MMP9 expression involves the ROS-dependent ERK-NF-κB signal pathways in VSMC. This discovery raises a new regulation pathway in the VSMC, and it shows the potential to help to find a new solution to treating aortic aneurysm and aortic dissection. |
| | |
Authors:
|
Hao Zhang; Zhi-Wei Wang; Hong-Bing Wu; Zhi Li; Luo-Cheng Li; Xiao-Ping Hu; Zong-Li Ren; Bai-Jun Li; Zhi-Peng Hu |
Related Documents
:
|
18583987 - Transcriptional regulation and function of the neurospora clock gene white collar 2 and... 20551177 - Flexible phase adjustment of circadian albumin d site-binding protein (dbp) gene expres... 15728337 - Low temperature induction of arabidopsis cbf1, 2, and 3 is gated by the circadian clock. 16783827 - Regulation of schistocerca gregaria neuroparsin transcript levels by juvenile hormone a... 18571497 - Differential expression and activation of rab27a in human eosinophils: relationship to ... 10050667 - The ctla-4 gene is expressed in placental fibroblasts. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-29 |
Journal Detail:
|
Title: Molecular and cellular biochemistry Volume: - ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2012-12-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Cardiothoracic Surgery, Renmin Hospital of Wuhan University, #99 Ziyang Road, Wuhan, 430060, Hubei, China, md_zhanghao@yahoo.cn. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of tumor suppressor PTEN gene on apoptosis and cell cycle of human airway smooth muscle cells...
Next Document: Rapidly progressive paraplegia and pleural empyema: how does that correlate?