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Transformed Hairy Roots of Discaria trinervis: A valuable tool for studying actinorhizal symbiosis in the context of intercellular infection.
MedLine Citation:
PMID:  21585269     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. Intercellular infection has been described in Discaria trinervis, an actinorhizal plant belonging to the Rosales order. To decipher the molecular mechanisms underlying intercellular infection with Frankia, we set up an efficient genetic transformation protocol for D. trinervis based on A. rhizogenes. We showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by Frankia strain BCU110501. Nitrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. In order to challenge the transformation system, the MtEnod11 promoter, a gene from Medicago truncatula widely used as a marker for early infection related symbiotic events in model legumes, was introduced in D. trinervis. MtEnod11:GUS expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. The ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen fixing root nodule symbioses.
Authors:
Leandro Imanishi; Alice Vayssières; Didier Bogusz; Claudine Franche; Luis Gabriel Wall; Sergio Svistoonoff
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-17
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  -     ISSN:  0894-0282     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Universidad Nacional de Quilmes, Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo (LBMIBS), Departamento de Ciencia y Tecnología, Bernal, Buenos Aires, Argentina; leandro_ima@yahoo.com.ar.
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