| Transformation of rat liver cell line by Rous sarcoma virus causes loss of cell surface fibronectin, accompanied with secretion of metallo-proteinase that preferentially digests the fibronectin. | |
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MedLine Citation:
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PMID: 2828345 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An epithelial cell line derived from the liver of a normal Buffalo rat (BRL) was transformed by Rous sarcoma virus (RSV). The RSV-transformed cells were separated into five clones (RSV-BRL1 through 5), which were morphologically different. RSV-BRL cells exhibited the following characteristics distinct from those of BRL cells: tumorigenicity, irregular cell arrangement, loose intercellular junction, growth in soft agar (anchorage-independent growth) except for RSV-BRL3 and 5, and loss of cell surface fibronectin. When BRL cells were cultured in the standard medium supplemented with the serum-free conditioned medium of RSV-BRL cells, the amount of the cell surface fibronectin decreased significantly. It was found that RSV-BRL cells secreted a proteinase capable of hydrolyzing the fibronectin, whereas BRL cells secreted hardly any of this proteinase. The fibronectin-hydrolyzing proteinase (FNase) could also hydrolyze plasma fibronectin added as an exogenous substrate. The hydrolysis of plasma fibronectin was inhibited by ethylenediamine tetraacetate, but stimulated by rho-chloromercuribenzoate and calcium ion. This indicates that FNase is a metallo-enzyme, but not a serine or thiol enzyme. In addition to the proteinase, RSV-BRL cells secreted plasminogen activator and a proteinase inhibitor which inhibited the activity of plasmin but not FNase. |
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Authors:
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K Miyazaki; Y Ashida; Y Kihira; K Mashima; J Yamashita; T Horio |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 102 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1987 Sep |
Date Detail:
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Created Date: 1988-03-07 Completed Date: 1988-03-07 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 569-82 Citation Subset: IM |
Affiliation:
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Institute for Protein Research, Osaka University. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Avian Sarcoma Viruses* Cell Line Cell Transformation, Viral* Cells, Cultured Chromatography, Affinity Colony-Forming Units Assay Electrophoresis, Polyacrylamide Gel Fibrinogen / analysis Fibronectins / immunology, metabolism* Peptide Hydrolases / metabolism, secretion* Plasminogen Activators / metabolism Rats |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 9001-32-5/Fibrinogen; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.-/Plasminogen Activators |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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