| Transformation of pleomorphic adenoma to carcinoma ex pleomorphic adenoma of the parotid gland is independent of p53 mutations. | |
| | |
MedLine Citation:
|
PMID: 19950209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND AND OBJECTIVES: This retrospective study was performed to evaluate the status of p53 in pleomorphic adenomas and carcinomas ex pleomorphic adenoma in the parotid gland. As loss or mutation of p53 can cause malignant transformation, the possible degeneration of pleomorphic adenomas to carcinomas ex pleomorhic adenoma was investigated by mutational analysis. METHODS: Twenty-five Patients including 14 patients with pleomorphic adenomas and 11 patients with carcinoma ex pleomorphic adenoma of the parotid gland were examined for p53 status. DNA was extracted out of paraffin-embedded tissue and PCR was performed for the coding exons 2-11. Denaturing gradient gel electrophoresis (DGGE) was carried out for mutational analysis and DNA sequencing was performed in case of a suspected mutation. RESULTS: Fourteen pleomorphic adenomas and 11 carcinomas ex pleomorphic adenoma were screened for p53 status and potent mutations. Subsequent sequencing of the distinct exons showed no mutation. CONCLUSION: We could not detect mutations of p53 neither in benign nor malignant parotid tumors and we therefore assume that p53 plays no role in the transformation from pleomorphic adenoma to carcinoma ex pleomorphic adenoma. |
| | |
Authors:
|
Claudia Gedlicka; Chike B Item; Markus W?gerbauer; Helga Martinek; Gregor Heiduschka; Boban M Erovic; Matthaeus Ch Grasl; Dietmar Thurnher |
Related Documents
:
|
15240859 - Longitudinal study on mutation profiles of core promoter and precore regions of the hep... 8176269 - Absence of p53 gene mutations in cutaneous melanoma. 9379679 - Exon 5 mutations in the p53 gene in relapsed childhood acute lymphoblastic leukemia. 8389669 - Germ line polymorphisms of p53 and cyp1a1 genes involved in human lung cancer. 21349879 - Evidence to support il-13 as a risk locus for psoriatic arthritis but not psoriasis vul... 10084119 - Sources of bias in the detection and reporting of p53 mutations in human cancer: analys... 20534169 - Association of tnf-alpha gene with spontaneous deep intracerebral hemorrhage in the tai... 3557109 - Patterns of somatic mutations in immunoglobulin variable genes. 2071019 - Analysis of coyote mitochondrial dna genotype frequencies: estimation of the effective ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of surgical oncology Volume: 101 ISSN: 1096-9098 ISO Abbreviation: J Surg Oncol Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-01-25 Completed Date: 2010-03-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0222643 Medline TA: J Surg Oncol Country: United States |
Other Details:
|
Languages: eng Pagination: 127-30 Citation Subset: IM |
Affiliation:
|
Department of Otorhinolaryngology, Medical University of Vienna, 1090 Vienna, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenoma, Pleomorphic
/
genetics* Adult Aged Aged, 80 and over Female Genes, p53 / genetics* Humans Male Middle Aged Parotid Neoplasms / genetics* Retrospective Studies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Non-resorbing osteoclasts induce migration and osteogenic differentiation of mesenchymal stem cells.
Next Document: Globally optimal, minimum stored energy, double-doughnut superconducting magnets.