| Transformation of leukotriene A4 to lipoxins by rat kidney mesangial cell. | |
| | |
MedLine Citation:
|
PMID: 2547366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Incubation of rat mesangial cells with leukotriene A4 in the presence of calcium ionophore A23187 led to a substrate dependent formation of lipoxin and its isomers. The major metabolite coeluted with authentic lipoxin A4 (LXA4) and lipoxin B4 (LXB4) in RP-HPLC system, and possessed a characteristic U.V. spectrum and C-value which were identical to authentic standards. GC/MS analysis on LXA4 further demonstrates that the mesangial cell derived LXA4 was identical to that reported by Serhan et al. (1) as LXA4 [5(S), 6,(R), 15(S)-trihydroxy7,9,13-trans-11-cis-eicosatetraenoic acid]. The formation of LXA4 was linear with substrate (LTA4) concentration. No similar products occurred in boiled controls. Incubation of mesangial cell with 15-HPETE failed to produce any lipoxin-like material. The absence of LX-like substance following incubation of 15-HPETE with mesangial cells suggested that 5-lipoxygenase activity is not expressed in mesangial cells under these conditions. The generation of LXA4 from LTA4 in mesangial cells suggested that there is an active 15- or 12- lipoxygenase activity in the kidney. The production of LX may play an important role in the regulation of renal function and the response to inflammatory stimuli. |
| | |
Authors:
|
R Garrick; S Y Shen; S Ogunc; P Y Wong |
Related Documents
:
|
3631536 - The structural relationship between mesangial cells and basement membrane of the renal ... 1922616 - A case of angiotropic large cell lymphoma manifesting nephrotic syndrome and treated su... 511606 - Cytochemical characterization of cultured adult, guinea pig glomerular cells. 16968946 - Eicosanoid transcellular biosynthesis: from cell-cell interactions to in vivo tissue re... 18493826 - Methodology for the formation of functional, cell-based cardiac pressure generation con... 19896826 - Multipotent skin-derived precursors: from biology to clinical translation. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 162 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 Jul |
Date Detail:
|
Created Date: 1989-09-07 Completed Date: 1989-09-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 626-33 Citation Subset: IM |
Affiliation:
|
Department of Medicine, New York Medical College, Valhalla 10595. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonate 5-Lipoxygenase / metabolism Arachidonic Acid Arachidonic Acids / metabolism Calcimycin / pharmacology Cells, Cultured Chromatography, High Pressure Liquid Glomerular Mesangium / drug effects, metabolism* Hydroxyeicosatetraenoic Acids / metabolism* Leukotriene A4 Leukotrienes / metabolism* Lipid Peroxides / metabolism Lipoxins* Male Mass Spectrometry Rats Rats, Inbred Strains Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
|
0/Arachidonic Acids; 0/Hydroxyeicosatetraenoic Acids; 0/Leukotriene A4; 0/Leukotrienes; 0/Lipid Peroxides; 0/Lipoxins; 0/lipoxin A4; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 67675-14-3/15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; 92950-25-9/lipoxin B4; EC 1.13.11.34/Arachidonate 5-Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Fetal and adult sheep adrenal cortical cells contain glucocorticoid receptors.
Next Document: Transcription of 5S RNA genes containing insertion mutation and the assembly of preinitiation comple...