Document Detail


Transformation of leukotriene A4 to lipoxins by rat kidney mesangial cell.
MedLine Citation:
PMID:  2547366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Incubation of rat mesangial cells with leukotriene A4 in the presence of calcium ionophore A23187 led to a substrate dependent formation of lipoxin and its isomers. The major metabolite coeluted with authentic lipoxin A4 (LXA4) and lipoxin B4 (LXB4) in RP-HPLC system, and possessed a characteristic U.V. spectrum and C-value which were identical to authentic standards. GC/MS analysis on LXA4 further demonstrates that the mesangial cell derived LXA4 was identical to that reported by Serhan et al. (1) as LXA4 [5(S), 6,(R), 15(S)-trihydroxy7,9,13-trans-11-cis-eicosatetraenoic acid]. The formation of LXA4 was linear with substrate (LTA4) concentration. No similar products occurred in boiled controls. Incubation of mesangial cell with 15-HPETE failed to produce any lipoxin-like material. The absence of LX-like substance following incubation of 15-HPETE with mesangial cells suggested that 5-lipoxygenase activity is not expressed in mesangial cells under these conditions. The generation of LXA4 from LTA4 in mesangial cells suggested that there is an active 15- or 12- lipoxygenase activity in the kidney. The production of LX may play an important role in the regulation of renal function and the response to inflammatory stimuli.
Authors:
R Garrick; S Y Shen; S Ogunc; P Y Wong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  162     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-09-07     Completed Date:  1989-09-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  626-33     Citation Subset:  IM    
Affiliation:
Department of Medicine, New York Medical College, Valhalla 10595.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonate 5-Lipoxygenase / metabolism
Arachidonic Acid
Arachidonic Acids / metabolism
Calcimycin / pharmacology
Cells, Cultured
Chromatography, High Pressure Liquid
Glomerular Mesangium / drug effects,  metabolism*
Hydroxyeicosatetraenoic Acids / metabolism*
Leukotriene A4
Leukotrienes / metabolism*
Lipid Peroxides / metabolism
Lipoxins*
Male
Mass Spectrometry
Rats
Rats, Inbred Strains
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Hydroxyeicosatetraenoic Acids; 0/Leukotriene A4; 0/Leukotrienes; 0/Lipid Peroxides; 0/Lipoxins; 0/lipoxin A4; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 67675-14-3/15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; 92950-25-9/lipoxin B4; EC 1.13.11.34/Arachidonate 5-Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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